CCND2 Human
Cyclin D2 Human Recombinant
Cat. No.
BT2274
Source
Escherichia Coli.
Synonyms
G1/S-specific cyclin-D2, CCND2, cyclin D2, KIAK0002.
Appearance
Sterile Filtered clear colorless solution.
Purity
Greater than 85% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description
CCND2 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 313 amino acids (1-289 a.a.) and having a molecular mass of 35.6kDa.
CCND2 is fused to a 24 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
CCND2 is fused to a 24 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Product Specs
Introduction
Cyclin D2, encoded by the CCND2 gene, is a crucial regulator of cell cycle progression. As a member of the cyclin family, CCND2 exhibits fluctuating protein levels throughout the cell cycle. Its primary role is to activate cyclin-dependent kinases (CDKs) 4 and 6, forming complexes essential for the transition from the G1 to S phase of the cell cycle. This transition is critical for DNA replication and cell division. CCND2 interacts with the retinoblastoma protein (Rb), a tumor suppressor, and participates in its phosphorylation. This phosphorylation inactivates Rb, facilitating cell cycle progression. Aberrant expression of CCND2 is implicated in various cancers, including ovarian and testicular tumors. Studies in mice have highlighted the importance of CCND2 in ovarian function, particularly in granulosa and germ cell proliferation.
Description
Recombinant human CCND2, expressed in E. coli, is available as a single, non-glycosylated polypeptide chain. This protein consists of 313 amino acids, encompassing residues 1 to 289 of the native CCND2 sequence, along with an N-terminal 24-amino acid His-tag for purification. The molecular weight of this recombinant CCND2 protein is 35.6 kDa. Purification is achieved through proprietary chromatographic techniques.
Physical Appearance
A clear, colorless solution that has been sterilized by filtration.
Formulation
The provided CCND2 protein solution has a concentration of 1 mg/ml and is formulated in a buffer consisting of 20 mM Tris-HCl (pH 8.0), 10% glycerol, 2 mM DTT, and 1 M Urea.
Stability
For short-term storage (up to 2-4 weeks), the CCND2 protein should be kept at 4°C. For extended storage, it is recommended to store the protein at -20°C. The addition of a carrier protein (0.1% HSA or BSA) is advised for long-term storage. It's important to avoid repeated freezing and thawing of the protein.
Purity
The purity of the CCND2 protein is determined to be greater than 85% based on SDS-PAGE analysis.
Synonyms
G1/S-specific cyclin-D2, CCND2, cyclin D2, KIAK0002.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSHMELLCH EVDPVRRAVR DRNLLRDDRV LQNLLTIEER YLPQCSYFKC VQKDIQPYMR RMVATWMLEV CEEQKCEEEV FPLAMNYLDR FLAGVPTPKS HLQLLGAVCM FLASKLKETS PLTAEKLCIY TDNSIKPQEL LEWELVVLGK LKWNLAAVTP HDFIEHILRK LPQQREKLSL IRKHAQTFIA LCATDFKFAM YPPSMIATGS VGAAICGLQQ DEEVSSLTCD ALTELLAKIT NTDVDCLKAC QEQIEAVLLN SLQQYRQDQR DGSKSEDELD QASTPTDVRD IDL.
Product Science Overview
Introduction
Cyclin D2 is a member of the highly conserved cyclin family, which plays a crucial role in regulating the cell cycle. Cyclins function as regulatory subunits for cyclin-dependent kinases (CDKs), and their periodic expression and degradation are essential for the temporal coordination of mitotic events .
Gene and Protein Structure
Function
Cyclin D2 is involved in several critical cellular processes:
- Cell Cycle Regulation: It regulates the G1/S transition by forming complexes with CDK4 or CDK6, leading to the phosphorylation of the retinoblastoma protein (Rb), which in turn allows the cell cycle to progress .
- Cell Proliferation: Cyclin D2 is essential for the proliferation of various cell types, including ovarian granulosa and germ cells .
- Tumor Suppression: It interacts with tumor suppressor proteins and is involved in their phosphorylation, which is crucial for controlling cell growth and preventing tumor formation .
Recombinant Cyclin D2
Recombinant Cyclin D2 is produced using E. coli expression systems and is often tagged with His-ABP for purification purposes . The recombinant form retains the functional properties of the native protein and is used in various research applications, including blocking assays and control experiments .
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