CCNA2 Human

Cyclin-A2 Human Recombinant
Cat. No.
BT2050
Source
Escherichia Coli.
Synonyms
Cyclin-A2, Cyclin-A, CCNA2, CCN1, CCNA.
Appearance
Sterile Filtered clear colorless solution.
Purity
Greater than 90% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

CCNA2 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 456 amino acids (1-432 a.a) and having a molecular mass of 51.1kDa.
CCNA2 is fused to a 24 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
CCNA2, a member of the cyclin family, plays a crucial role in regulating the cell cycle. Its protein levels fluctuate cyclically, coordinating cell cycle events. Unlike cyclin A1, found only in germ cells, CCNA2 is ubiquitously expressed. By binding and activating CDC2 or CDK2 kinases, CCNA2 drives cell cycle progression through the G1/S and G2/M transitions.
Description
This recombinant CCNA2 protein, expressed in E. coli, is a single, non-glycosylated polypeptide chain. It consists of 456 amino acids (residues 1-432), resulting in a molecular weight of 51.1 kDa. The protein includes a 24-amino acid His-tag at the N-terminus to facilitate purification using proprietary chromatographic techniques.
Physical Appearance
A clear, colorless solution that has been sterilized through filtration.
Formulation
The CCNA2 protein is supplied in a solution with a concentration of 0.5 mg/ml. The solution also contains 20 mM Tris-HCl buffer at a pH of 8.0, 20% glycerol, 0.15 M NaCl, and 1 mM DTT.
Stability
For short-term storage (up to 2-4 weeks), the protein can be stored at 4°C. For longer-term storage, it is recommended to freeze the protein at -20°C. To further enhance long-term stability, consider adding a carrier protein like HSA or BSA (0.1%). It's crucial to avoid repeated freezing and thawing of the protein.
Purity
Purity analysis using SDS-PAGE has determined that the protein is greater than 90% pure.
Synonyms
Cyclin-A2, Cyclin-A, CCNA2, CCN1, CCNA.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSHMLGNSA PGPATREAGS ALLALQQTAL QEDQENINPE KAAPVQQPRT RAALAVLKSG NPRGLAQQQR PKTRRVAPLK DLPVNDEHVT VPPWKANSKQ PAFTIHVDEA EKEAQKKPAE SQKIEREDAL AFNSAISLPG PRKPLVPLDY PMDGSFESPH
TMDMSIVLED EKPVSVNEVP DYHEDIHTYL REMEVKCKPK VGYMKKQPDI TNSMRAILVD WLVEVGEEYK LQNETLHLAV NYIDRFLSSM SVLRGKLQLV GTAAMLLASK FEEIYPPEVA EFVYITDDTY TKKQVLRMEH LVLKVLTFDL AAPTVNQFLT QYFLHQQPAN CKVESLAMFL
GELSLIDADP YLKYLPSVIA GAAFHLALYT VTGQSWPESL IRKTGYTLES LKPCLMDLHQ TYLKAPQHAQ QSIREKYKNS KYHGVSLLNP PETLNL.

Product Science Overview

Introduction

Cyclin-A2, also known as CCNA2, is a member of the highly conserved cyclin family, which plays a crucial role in cell cycle regulation. Cyclin-A2 is ubiquitously expressed in various tissues and is involved in the regulation of both the S phase and G2/M transition of the cell cycle .

Structure and Expression

Cyclin-A2 is a protein that activates cyclin-dependent kinases (CDKs), specifically CDK2 and CDK1, to promote cell cycle progression. The human recombinant form of Cyclin-A2 is often expressed in systems such as Escherichia coli or baculovirus-insect cells to study its function and for use in various research applications .

Function

Cyclin-A2 is essential for the initiation and progression of DNA replication during the S phase. It also plays a critical role in the G2/M transition, ensuring that cells properly enter mitosis. Cyclin-A2 achieves this by binding to and activating CDK2 during the S phase and CDK1 during the G2/M transition .

Recombinant Expression

The recombinant expression of human Cyclin-A2 has been a challenge due to issues related to solubility, yield, and purity. However, optimized conditions have been developed to produce sufficient quantities of soluble and active Cyclin-A2. This involves the use of molecular chaperones and specific expression systems to ensure proper folding and functionality of the protein .

Applications

Recombinant Cyclin-A2 is used in various research applications, including:

  • Crystallization and Structural Studies: To understand the protein’s structure and its interaction with other molecules.
  • Drug Discovery: To identify potential inhibitors that can target the cyclin-CDK complex, which is crucial for developing therapies for diseases such as cancer.
  • Biological Activity Assays: To study the protein’s function and its role in cell cycle regulation .
Clinical Significance

Cyclin-A2 is significantly overexpressed in various cancer types and has been identified as a biomarker for the prognosis of estrogen receptor-positive (ER+) breast cancer. It is also associated with tamoxifen resistance, making it a critical target for cancer research and treatment .

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THE BIOTEK
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