Zika NS1
Zika NS1 Protein Recombinant
Cat. No.
BT11286
Source
Escherichia Coli.
Synonyms
Appearance
Sterile Filtered solution.
Purity
Zika NS1 protein is >95% pure as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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In Stock 
Description
The E.Coli derived Recombinant Zika NS1 protein (Strain: ZikaSPH2015) having an Mw of 45kDa is derived from the full length Zika NS1 protein.
The Zika NS1 protein is fused to a 6xHis tag at C-terminus and purified by proprietary chromatographic technique.
The Zika NS1 protein is fused to a 6xHis tag at C-terminus and purified by proprietary chromatographic technique.
Product Specs
Introduction
Zika virus (ZIKV) is a member of the Flaviviridae family and the Flavivirus genus, transmitted primarily through the bite of infected Aedes mosquitoes, particularly A. aegypti and A. albopictus. These mosquitoes are known to be active during daylight hours. Zika virus shares similarities with other flaviviruses such as dengue, yellow fever, Japanese encephalitis, and West Nile viruses. Like its counterparts, the Zika virus possesses an envelope, an icosahedral shape, and a nonsegmented, single-stranded RNA genome with a positive-sense orientation. Infection with Zika virus typically results in Zika fever, a disease that is often asymptomatic or presents with mild symptoms resembling a mild form of dengue fever. Rest is the generally recommended treatment for Zika fever. Recent studies, particularly since February 2016, have provided increasing evidence suggesting a possible link between Zika fever during pregnancy and abnormal brain development in the fetus. This transmission is thought to occur from mother to child and may lead to complications such as miscarriage or microcephaly, a condition characterized by an abnormally small head size. However, it is important to note that the causative relationship between Zika virus and microcephaly has not yet been definitively established. Furthermore, a connection has been observed between Zika virus infection in adults and neurological complications, including Guillain-Barré syndrome, a disorder affecting the nervous system. Historically, from the 1950s onward, Zika virus was primarily confined to a narrow equatorial belt spanning Africa and Asia. However, between 2013 and 2014, the virus began to spread eastward across the Pacific Ocean, reaching French Polynesia, New Caledonia, the Cook Islands, and Easter Island. By 2015, Zika virus had reached Mexico, Central America, the Caribbean, and South America, with the outbreak in South America reaching pandemic levels.
Description
The Zika NS1 protein is a recombinant protein derived from E. coli and based on the ZikaSPH2015 strain of the Zika virus. It has a molecular weight of 45 kDa and is produced from the full-length Zika NS1 protein sequence. For purification purposes, a 6xHis tag is attached to the C-terminus of the protein. The purification process involves proprietary chromatographic techniques.
Physical Appearance
Sterile solution that has been passed through a filter.
Formulation
The Zika NS1 protein is supplied in a solution containing phosphate-buffered saline (PBS) and 100mM arginine.
Stability
While the Zika NS1 protein remains stable for a week when stored at 4°C, it is recommended to store it at a temperature below -18°C to ensure long-term stability. Repeated freeze-thaw cycles should be avoided.
Purity
Analysis by SDS-PAGE indicates that the purity of the Zika NS1 protein is greater than 95%.
Applications
The optimal working titer of the Zika NS1 protein may vary depending on the specific application and should be determined empirically by each laboratory.
Source
Escherichia Coli.
Product Science Overview
Introduction
The Zika virus (ZIKV) is a mosquito-borne flavivirus that has caused significant outbreaks in recent years, particularly in the South Pacific, Americas, and Caribbean islands. ZIKV infection in humans can lead to severe neurological disorders and congenital abnormalities, such as microcephaly . One of the key proteins involved in the Zika virus’s life cycle and pathogenicity is the non-structural protein 1 (NS1).
Structure and Function of NS1
NS1 is a glycoprotein that plays a crucial role in the replication and pathogenesis of flaviviruses, including ZIKV. It exists in different forms within the host cell:
Recombinant Zika NS1 Protein
Recombinant Zika NS1 protein is produced using various expression systems, such as Vero cells or human embryonic kidney cells (HEK293). These systems are transduced with a vector containing the NS1 gene from a ZIKV strain. The recombinant protein is then purified and used for various applications, including:
- Vaccine Development: Recombinant NS1 protein is highly immunogenic and can induce the production of antibodies that specifically target the NS1 dimer. This makes it a potential candidate for vaccine development .
- Diagnostic Tools: Due to its immunogenic properties, NS1 can be used as a biomarker for diagnosing ZIKV infections .
- Research: Recombinant NS1 protein is used in research to study the virus’s life cycle, pathogenic mechanisms, and interactions with the host immune system .
Applications and Benefits
The use of recombinant Zika NS1 protein has several advantages:
- High Purity: Recombinant proteins can be produced with high purity, ensuring consistent quality for research and clinical applications .
- Specificity: The antibodies generated against recombinant NS1 are highly specific, allowing for accurate detection and study of ZIKV .
- Versatility: Recombinant NS1 protein can be used in various formats, such as carrier-free formulations, making it suitable for different experimental setups .
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