Zika NS1 Paired Antibody

Zika virus (ZIKV) is a member of the Flaviviridae family and the Flavivirus genus. It is primarily transmitted by Aedes mosquitoes, such as Aedes aegypti and Aedes albopictus . The virus is related to other flaviviruses, including dengue, yellow fever, Japanese encephalitis, and West Nile viruses . Zika virus has a nonsegmented, single-stranded, positive-sense RNA genome and is enveloped and icosahedral in structure .

The nonstructural protein 1 (NS1) of Zika virus plays a crucial role in viral replication and immune evasion. NS1 is a glycoprotein that is secreted from infected cells and can be found in the blood of infected individuals. It is involved in immune modulation and has been implicated in the pathogenesis of Zika virus infection .

Mouse anti-Zika NS1 paired antibodies are monoclonal antibodies specifically designed to target the NS1 protein of Zika virus. These antibodies are produced using recombinant full-length NS1 protein as the immunogen, typically expressed in HEK293 cells . The antibodies are purified using protein G or protein A chromatography to ensure high purity and specificity .

Mouse anti-Zika NS1 paired antibodies are commonly used in various immunoassays, including sandwich ELISA (enzyme-linked immunosorbent assay). In sandwich ELISA, one antibody is used as the capture antibody, while the other is used as the detection antibody . This pairing allows for the sensitive and specific detection of Zika virus NS1 protein in biological samples.

These antibodies demonstrate high specificity for Zika virus NS1 protein and negligible cross-reactivity with NS1 proteins from other flaviviruses, such as dengue virus, Japanese encephalitis virus, and yellow fever virus . However, a small amount of cross-reactivity has been observed with the NS1 protein from West Nile virus in direct ELISA .

The development of mouse anti-Zika NS1 paired antibodies has been instrumental in advancing research and diagnostics related to Zika virus. These antibodies enable the accurate detection and quantification of NS1 protein, which is essential for understanding the pathogenesis of Zika virus infection and for developing diagnostic assays.

In research, these antibodies have been used to study the immune response to Zika virus infection and to evaluate the efficacy of potential vaccines . For example, studies have shown that antibodies elicited by an NS1-based vaccine can protect mice against lethal challenge by Zika virus . Additionally, passive transfer of immune sera containing NS1-specific antibodies has been shown to provide significant protection against Zika virus infection in animal models .