Zika Envelope Antibody

Polyclonal Rabbit Anti Zika envelope
Cat. No.
BT5072
Source
Synonyms
Appearance
Sterile Filtered clear colorless solution.
Purity
Pure Rabbit IgG.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

Zika virus Full length envelope DNA was cloned into an expression vector containing a recombinant mammalian cell promoter vector which was injected into rabbit for in vivo expression of Zika envelope inducing the production of the specific Zika envelope antibody in rabbit. High titer anti-Zika envelope antibody was detected, dilute 1:4000 to 5000 before use.

Product Specs

Introduction
Zika virus (ZIKV), a member of the Flaviviridae family and Flavivirus genus, is primarily transmitted by diurnal Aedes mosquitoes, including A. aegypti and A. albopictus. ZIKV shares genetic similarities with dengue, yellow fever, Japanese encephalitis, and West Nile viruses. Like other flaviviruses, Zika possesses an enveloped, icosahedral structure and contains a non-segmented, single-stranded, positive-sense RNA genome. Zika fever, the infection caused by the virus, often presents with no or mild symptoms, resembling a mild form of dengue fever, and typically necessitates rest for treatment. Since February 2016, growing evidence suggests a link between Zika fever in pregnant women and fetal brain abnormalities, potentially leading to miscarriage or microcephaly, through mother-to-child transmission. However, a definitive causal relationship between Zika virus and microcephaly remains unestablished. Additionally, a connection has been identified between Zika infection in adults and neurological complications, such as Guillain-Barré syndrome.
Description
To generate Zika envelope antibodies, the full-length Zika virus envelope DNA was cloned into a mammalian cell expression vector containing a recombinant promoter. This construct was then used to immunize rabbits, leading to the in vivo expression of the Zika envelope protein and subsequent production of specific antibodies. The resulting rabbit serum exhibited high titers of anti-Zika envelope antibodies, typically requiring a dilution of 1:4000 to 1:5000 before use.
Purity
The antibody solution consists of purified rabbit IgG.
Physical Appearance
The antibody solution is a clear, colorless, and sterile-filtered liquid.
Formulation
The antibody is supplied as a protein solution in phosphate-buffered saline (PBS) with 0.1M Glycine, pH 8.0.
Stability
For short-term storage (up to 2-4 weeks), the antibody should be stored at 4°C. For long-term storage, freezing at -20°C is recommended. To enhance stability during prolonged storage, the addition of a carrier protein (0.1% HSA or BSA) is advisable. Repeated freeze-thaw cycles should be avoided.
Purification Method

Purified by affinity chromatographic technique.

Product Science Overview

Introduction

The Zika virus, a member of the Flaviviridae family, has garnered significant attention due to its association with severe neurological disorders, including microcephaly in newborns and Guillain-Barré syndrome in adults. The envelope protein (E protein) of the Zika virus plays a crucial role in the virus’s ability to infect host cells and is a primary target for antibody development. Polyclonal Rabbit Anti-Zika Envelope antibodies are widely used in research to detect and study the Zika virus envelope protein.

Production of Polyclonal Rabbit Anti-Zika Envelope Antibody

Polyclonal antibodies are produced by immunizing an animal, in this case, a rabbit, with an antigen—in this instance, the Zika virus envelope protein. The rabbit’s immune system responds by producing a variety of antibodies that recognize multiple epitopes on the antigen. The serum containing these antibodies is then collected and purified to obtain the polyclonal antibody preparation.

Characteristics and Applications

Polyclonal Rabbit Anti-Zika Envelope antibodies are characterized by their ability to recognize multiple epitopes on the Zika virus envelope protein. This multi-epitope recognition enhances their sensitivity and makes them suitable for various applications, including:

  • Western Blotting (WB): Used to detect the presence of the Zika virus envelope protein in a sample.
  • Immunocytochemistry (ICC): Allows for the visualization of the Zika virus envelope protein within cells.
  • Immunohistochemistry (IHC): Used to detect the Zika virus envelope protein in tissue samples.
  • Enzyme-Linked Immunosorbent Assay (ELISA): Quantifies the amount of Zika virus envelope protein in a sample.
  • Immunoprecipitation (IP): Isolates the Zika virus envelope protein from a mixture of proteins.
Advantages of Using Polyclonal Rabbit Anti-Zika Envelope Antibody
  1. High Sensitivity: Due to the recognition of multiple epitopes, polyclonal antibodies often have higher sensitivity compared to monoclonal antibodies.
  2. Versatility: Polyclonal antibodies can be used in a wide range of applications, making them a versatile tool in research.
  3. Cost-Effective: The production of polyclonal antibodies is generally less expensive than monoclonal antibodies.
Limitations

Despite their advantages, polyclonal antibodies also have some limitations:

  • Batch-to-Batch Variability: Since polyclonal antibodies are derived from different animals, there can be variability between different batches.
  • Cross-Reactivity: Polyclonal antibodies may recognize epitopes on proteins other than the target antigen, leading to potential cross-reactivity.

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