Vimentin Antibody

Vimentin, Mouse Anti Human Antibody
Cat. No.
BT2975
Source
Synonyms
Appearance
Purity
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

Product Specs

Introduction
Vimentin, a type III intermediate filament protein, plays a crucial role in various cellular processes, including cell adhesion, migration, and signaling. Its expression is often dysregulated in cancer, contributing to tumor progression and metastasis. For instance, in malignant glioma cells, vimentin expression is influenced by cellular density, drug delivery methods, and chemo/radiotherapy. The protein, along with detyrosinated microtubules, provides structural integrity to microtentacles found on detached tumor cells, facilitating their spread. In colorectal cancer, vimentin overexpression is linked to aberrant Notch and TGF-beta signaling pathways. Additionally, vimentin serves as a substrate for transglutaminases in arteries, and its dimerization through these enzymes may regulate vasodilation and vascular remodeling. Studies have demonstrated that suppressing vimentin expression can hinder the migration and invasion of colon and breast cancer cell lines, highlighting its potential as a therapeutic target.
Formulation
The antibody is supplied as a 1 mg/ml solution in phosphate-buffered saline (PBS) following reconstitution.
Shipping Conditions
The antibody is shipped in a lyophilized form at ambient temperature.
Storage Procedures
For long-term storage, keep the lyophilized antibody at 4°C in a dry environment. After reconstitution, aliquot the antibody and store at -20°C if not intended for use within one month.
Solubility
To reconstitute, add sterile water (H2O) to the lyophilized antibody. Mix the solution gently, ensuring to wash the sides of the vial. Allow 30-60 seconds for complete reconstitution before use.
Titer
For intracellular staining using flow cytometry, a concentration of 5-10 µl of antibody per 1,000,000 cells is recommended. Ensure to wash the cells twice with PBS containing sodium azide (PBS/Azide) before adding the appropriate secondary fluorescent reagent.
Applications
This Vimentin antibody is suitable for various applications, including direct enzyme-linked immunosorbent assay (ELISA), Western blotting, immunoprecipitation, and immunohistochemistry.
Cross Reactivity
The Vimentin antibody exhibits broad cross-reactivity, recognizing Vimentin from various mammalian species except for mouse and rat.
Available Conjugates
For added convenience and flexibility in experimental design, the Vimentin antibody is also offered in conjugated forms, including biotinylated and fluorescein isothiocyanate (FITC)-conjugated versions.
Purification Method
Ion exchange column.
Type
Mouse Anti Human Monoclonal Antibody.
Clone
hVimentin.
Immunogen
Purified Human Vimentin.
Ig Subclass
mouse IgG1.

Product Science Overview

Introduction

Vimentin is a type of intermediate filament protein that is predominantly expressed in mesenchymal cells. It plays a crucial role in maintaining cell integrity, stabilizing cytoskeletal interactions, and supporting cellular processes such as migration and signaling. The mouse anti-human vimentin antibody is a monoclonal antibody specifically designed to detect human vimentin in various research applications.

Vimentin: Structure and Function

Vimentin is a 57 kDa protein that forms part of the cytoskeleton, which also includes microfilaments and microtubules. It is involved in maintaining cell shape, integrity, and stability. Vimentin filaments are dynamic structures that undergo rapid reorganization in response to cellular signals, aiding in processes such as wound healing, cell migration, and division .

Expression and Role in Development

Vimentin is expressed in cells of mesenchymal origin, including fibroblasts, endothelial cells, and certain types of immune cells. During embryonic development, vimentin is one of the first intermediate filaments to be expressed, playing a pivotal role in the differentiation and migration of cells. In adult tissues, vimentin expression is often associated with cells that have high plasticity and regenerative capacity .

Clinical Relevance

Vimentin is a marker for mesenchymal cells and is used in research to identify and study these cells in various tissues. Its expression is also examined in the context of epithelial-mesenchymal transition (EMT), a process implicated in cancer metastasis. Vimentin is often upregulated in metastatic cancer cells, making it a valuable marker for studying cancer progression and metastasis .

Mouse Anti-Human Vimentin Antibody

The mouse anti-human vimentin antibody is a monoclonal antibody that specifically binds to human vimentin. It is produced by immunizing mice with purified human vimentin protein, resulting in the generation of a highly specific antibody. This antibody is widely used in research applications such as Western blotting, immunohistochemistry, immunocytochemistry, and flow cytometry .

Applications in Research
  1. Western Blotting: The mouse anti-human vimentin antibody is used to detect vimentin protein levels in cell and tissue lysates. It provides a reliable tool for studying vimentin expression and its regulation under various experimental conditions.
  2. Immunohistochemistry: This antibody is used to visualize vimentin expression in tissue sections, allowing researchers to study the distribution and localization of vimentin in different tissues and under different pathological conditions.
  3. Immunocytochemistry: The antibody is used to stain cultured cells, enabling the study of vimentin organization and dynamics within cells.
  4. Flow Cytometry: The mouse anti-human vimentin antibody can be used to analyze vimentin expression in cell populations, providing insights into the role of vimentin in various cellular processes .

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THE BIOTEK
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