Vimentin Antibody, Biotin

Vimentin is a type III intermediate filament protein that is predominantly expressed in mesenchymal cells. It plays a crucial role in maintaining cell integrity, stabilizing cytoskeletal interactions, and supporting cellular processes such as migration, shape, and organelle positioning . The VIM gene, which encodes vimentin, is located on chromosome 10 in humans . Vimentin is often used as a marker to identify cells of mesenchymal origin and is involved in various biological processes, including intermediate filament organization, gene expression regulation, and cellular response to external stimuli .

Mouse anti-human antibodies are secondary antibodies produced by immunizing mice with human immunoglobulins. These antibodies are highly specific for human immunoglobulins and are commonly used in research and diagnostic applications to detect, sort, or purify human proteins . They are often conjugated with various labels, such as enzymes or fluorophores, to facilitate detection in techniques like ELISA, Western blotting, and immunohistochemistry . The development of mouse anti-human antibodies has significantly advanced biomedical research by enabling precise targeting and visualization of human proteins in various experimental settings .

Biotin, also known as vitamin B7 or vitamin H, is a water-soluble B-vitamin that plays a vital role in various metabolic processes. It acts as a coenzyme for carboxylase enzymes, which are involved in the synthesis of fatty acids, gluconeogenesis, and the catabolism of amino acids . Biotin is essential for maintaining healthy skin, hair, and nails, and it supports the normal functioning of the nervous system . It is found in a wide range of foods, including eggs, nuts, and certain vegetables, and is also produced by gut bacteria . Biotin deficiency is rare but can lead to symptoms such as hair loss, skin rashes, and neurological issues .

The combination of vimentin, mouse anti-human antibodies, and biotin is particularly useful in various research and diagnostic applications. For instance, biotinylated mouse anti-human vimentin antibodies can be used in immunoassays to detect vimentin expression in human tissues. This approach leverages the high specificity of mouse anti-human antibodies and the strong binding affinity of biotin to streptavidin, enabling sensitive and accurate detection of target proteins.