Vimentin PAT5H8AT Antibody

Vimentin PAT5H8AT, Mouse Anti Human
Cat. No.
BT3185
Source
Synonyms
Vimentin, Vim, FLJ36605.
Appearance
Sterile filtered colorless solution.
Purity
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

Product Specs

Introduction
Vimentin expression in human malignant glioma cells is influenced by factors like cellular density, drug delivery methods, and chemo/radiotherapy treatment. Vimentin and detyrosinated microtubules play a crucial role in providing structural support for the microtentacles observed in detached tumor cells, potentially facilitating metastatic spread. Notably, primary colorectal carcinomas exhibit abnormal vimentin expression and activated Notch and TGF-beta signaling pathways. Vimentin serves as a significant arterial substrate for transglutaminases, with transglutaminase-mediated vimentin dimerization offering a potential mechanism for regulating vasodilatory and remodeling responses. Studies have shown that inhibiting vimentin expression can hinder the migration and invasion of colon and breast cancer cell lines. As the primary intermediate filament protein in mesenchymal cells, vimentin holds value in differentially diagnosing undifferentiated neoplasms.
Physical Appearance
A clear, sterile solution without any color.
Formulation
The solution contains 1mg/ml of Vimentin antibody in a buffer consisting of PBS at pH 7.4, 10% Glycerol, and 0.02% Sodium Azide.
Storage Procedures
For short-term storage (up to 1 month), keep at 4°C. For longer storage, store at -20°C. Avoid repeated freezing and thawing.
Stability / Shelf Life
The product is stable for 12 months when stored at -20°C and for 1 month at 4°C.
Applications
This Vimentin antibody has undergone testing using various methods such as ELISA, Western blot, Flow cytometry, and ICC/IF to ensure its specificity and reactivity. However, it's important to note that optimal working concentrations may vary depending on the specific application, and users are advised to determine the optimal dilution for their experiments.
Synonyms
Vimentin, Vim, FLJ36605.
Purification Method
Vimentin antibody was purified from mouse ascitic fluids by protein-A affinity chromatography.
Type
Mouse Anti Human Monoclonal.
Clone
PAT5H8AT.
Immunogen
Anti-human Vimentin mAb, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human Vimentin protein 1-466 amino acids purified from E. coli.
Ig Subclass
Mouse IgG1 heavy chain and k light chain.

Product Science Overview

Introduction

Vimentin is a type III intermediate filament protein that is widely expressed in mesenchymal cells. It plays a crucial role in maintaining cell integrity, stabilizing cytoskeletal interactions, and supporting cellular processes such as migration and signaling. The Vimentin PAT5H8AT, Mouse Anti Human antibody is a monoclonal antibody specifically designed to target and bind to human vimentin.

Structure and Function of Vimentin

Vimentin is composed of a central α-helical rod domain flanked by non-helical head and tail domains. This structure allows vimentin to form coiled-coil dimers, which further assemble into tetramers and ultimately into intermediate filaments. These filaments provide mechanical support to cells and are involved in various cellular functions, including:

  • Cell Shape Maintenance: Vimentin filaments help maintain the shape and rigidity of cells.
  • Cell Migration: Vimentin is essential for the migration of cells during wound healing and embryonic development.
  • Signal Transduction: Vimentin interacts with various signaling molecules, influencing cellular responses to external stimuli.
Vimentin in Disease and Research

Vimentin is often upregulated in epithelial-mesenchymal transition (EMT), a process implicated in cancer metastasis. Its expression is also associated with various diseases, including:

  • Cancer: Vimentin is a marker for tumor progression and metastasis in several cancers, such as breast and colon cancer .
  • Cardiovascular Diseases: Vimentin plays a role in vascular remodeling and arterial stiffness.
  • Neurodegenerative Diseases: Abnormal vimentin expression is observed in conditions like Alzheimer’s disease.
Vimentin PAT5H8AT, Mouse Anti Human Antibody

The Vimentin PAT5H8AT antibody is a monoclonal antibody derived from mouse and is specifically designed to target human vimentin. This antibody is used in various scientific applications, including:

  • Western Blotting: To detect vimentin protein levels in cell and tissue samples.
  • Immunohistochemistry: To visualize vimentin expression in tissue sections.
  • Flow Cytometry: To analyze vimentin expression in cell populations.
  • Immunocytochemistry: To study vimentin distribution within cells.
Applications and Benefits

The Vimentin PAT5H8AT antibody is valuable in research due to its high specificity and affinity for human vimentin. It is used in studies related to:

  • Cancer Research: Investigating the role of vimentin in tumor progression and metastasis.
  • Cell Biology: Understanding the dynamics of the cytoskeleton and cell migration.
  • Disease Mechanisms: Exploring the involvement of vimentin in various diseases and identifying potential therapeutic targets.

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