Vimentin Antibody, FITC

Vimentin is a type III intermediate filament protein that is widely expressed in mesenchymal cells. It plays a crucial role in maintaining cell integrity, stabilizing cytoskeletal interactions, and supporting cellular processes such as migration and signaling . Vimentin is encoded by the VIM gene located on chromosome 10 in humans . It is involved in various biological processes, including intermediate filament organization, cellular response to stress, and regulation of gene expression .

During epithelial-mesenchymal transition (EMT), vimentin expression is upregulated, which is essential for processes like wound healing, embryogenesis, and cancer metastasis . Vimentin serves as a marker for mesenchymal cells and is often used in research to study cell differentiation and tumor progression .

Mouse anti-human antibodies are monoclonal antibodies produced by immunizing mice with human antigens. These antibodies are highly specific and are used extensively in research and clinical diagnostics . The development of mouse anti-human antibodies involves the fusion of mouse myeloma cells with spleen cells from immunized mice, creating hybridoma cells that produce the desired antibody .

One of the significant applications of mouse anti-human antibodies is in immunohistochemistry (IHC) and flow cytometry, where they are used to detect specific human proteins in tissue samples . These antibodies can also be conjugated with various labels, such as enzymes or fluorophores, to facilitate detection and quantification .

FITC, or Fluorescein Isothiocyanate, is a fluorescent dye commonly used in biological research for labeling proteins, antibodies, and other biomolecules . FITC has a high absorption rate and excellent fluorescence quantum yield, making it an ideal choice for various fluorescence-based assays . It emits bright yellow-green fluorescence when excited at a wavelength of 494 nm and has an emission peak at 520 nm .

The isothiocyanate group of FITC reacts with primary amines on proteins, forming stable thiourea bonds . This property allows FITC to be used in a wide range of applications, including flow cytometry, fluorescence microscopy, and immunofluorescence .

The combination of vimentin, mouse anti-human antibody, and FITC creates a powerful tool for studying cellular processes and protein localization. The mouse anti-human vimentin antibody specifically binds to human vimentin, allowing researchers to detect and visualize vimentin expression in human cells . When conjugated with FITC, the antibody can be used in fluorescence-based assays to provide detailed insights into vimentin distribution and dynamics within cells .

This conjugate is particularly useful in cancer research, where vimentin expression is often associated with tumor progression and metastasis . By using FITC-labeled mouse anti-human vimentin antibodies, researchers can track changes in vimentin expression during EMT and other critical cellular events .