Vimentin Bovine

Bovine Vimentin
Cat. No.
BT25628
Source
Bovine Lens.
Synonyms
Vimentin, Vim, FLJ36605.
Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Purity
Greater than 98.0% as determined by SDS-PAGE.
Usage
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

Bovine Vimentin protein produced in Bovine Lens, having a molecular weight of 57 kDa.

Product Specs

Introduction
Vimentin is a crucial intermediate filament protein found in mesenchymal cells, playing a vital role in various cellular processes, including structural support, cell signaling, and metastasis. Its expression in cancer cells is often associated with tumor progression and invasiveness. Vimentin's role in maintaining microtentacle structure in detached tumor cells contributes to metastatic spread. Furthermore, its aberrant expression in colorectal carcinomas is linked to activated Notch and TGF-beta signaling pathways. Vimentin also serves as a substrate for transglutaminases in arteries, influencing vasodilation and remodeling. Due to its role in mesenchymal cells, vimentin is a valuable marker for diagnosing undifferentiated neoplasms.
Description
Bovine Vimentin protein, with a molecular weight of 57 kDa, produced in Bovine Lens.
Physical Appearance
Sterile Filtered White lyophilized powder.
Formulation
The protein was lyophilized from a solution of 1 mg/ml containing 10mM sodium phosphate (pH 7.5), 6M urea, 2mM DTT, 1mM EDTA, and 10mM methylammonium chloride.
Solubility
Reconstitute the lyophilized Bovine Vimentin in sterile 18MΩ-cm H2O to a concentration of at least 100µg/ml. This solution can be further diluted in other aqueous solutions.
Stability
Lyophilized Bovine Vimentin remains stable at room temperature for up to 3 weeks. However, for long-term storage, it is recommended to store it desiccated below -18°C. Upon reconstitution, Bovine Vimentin should be stored at 4°C for a period of 2-7 days. For extended storage, freeze at -18°C. Avoid repeated freeze-thaw cycles. Adding a carrier protein like HSA or BSA (0.1%) can enhance long-term storage stability.
Purity
Purity exceeds 98.0% as determined by SDS-PAGE analysis.
Reconstitution To Filaments
To obtain protofilaments and filament complexes, first dissolve the vimentin in the provided 6M urea buffer. Next, dialyze the polypeptide solution in a stepwise manner, first against a solution of 4M urea and subsequently against a low salt buffer (50mM NaCl, 2mM dithiothreitol, 10mM Sodium Phosphate, pH 7.4). For immunization purposes, further dialysis against PBS (phosphate buffered saline, such as Dulbecco's PBS) may be performed. References: - Hatzfeld M. and Franke W.W. (1985). J. Cell Biol. 101, 1826-1841. - Hatzfeld M. et al. (1987). J. Mol. Biol. 197, 237-255.
Synonyms
Vimentin, Vim, FLJ36605.
Source
Bovine Lens.

Product Science Overview

Structure and Properties

Bovine vimentin, like other vimentin proteins, is composed of a central α-helical rod domain flanked by non-helical head and tail domains. This structure allows vimentin to form coiled-coil dimers, which further assemble into tetramers and ultimately into intermediate filaments. The molecular weight of bovine vimentin is approximately 57 kDa .

Function

Vimentin is essential for several cellular functions:

  • Structural Support: It provides mechanical support to cells, helping them withstand stress.
  • Intracellular Transport: Vimentin is believed to be involved in the transport of proteins between the nucleus and the plasma membrane .
  • Cell Migration: It plays a role in cell migration, which is crucial during wound healing and embryonic development.
  • Signal Transduction: Vimentin interacts with various signaling molecules, influencing cell signaling pathways.
Expression and Regulation

Vimentin is developmentally regulated and is expressed during the early stages of cellular development. It is co-expressed with desmin in certain cell types. The expression of vimentin can be influenced by cellular density, drug delivery algorithms, and treatments such as chemotherapy and radiotherapy .

Clinical Significance

Vimentin has significant implications in medical research and diagnostics:

  • Cancer Research: Aberrant expression of vimentin is observed in various cancers, including colorectal and breast cancers. It is associated with increased cell migration and invasion, contributing to metastasis .
  • Disease Diagnosis: Vimentin is used as a marker in the differential diagnosis of undifferentiated neoplasms due to its presence in mesenchymal cells .
  • Therapeutic Target: Targeting vimentin expression or function could potentially inhibit cancer cell migration and invasion, offering a therapeutic strategy for cancer treatment.
Research and Applications

Bovine vimentin is widely used in research to study its role in cellular processes and disease mechanisms. Recombinant bovine vimentin protein is produced and utilized in various laboratory applications, including immunization and structural studies .

In conclusion, bovine vimentin is a vital intermediate filament protein with diverse roles in cellular structure, function, and disease. Its study continues to provide valuable insights into cell biology and potential therapeutic approaches for various diseases.

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