VEGF Human, His

Vascular Endothelial Growth Factor Human Recombinant, His
Cat. No.
BT7614
Source
Escherichia Coli.
Synonyms
Vascular endothelial growth factor A, VEGF-A, Vascular permeability factor, VPF, VEGF, MGC70609.
Appearance
Sterile Filtered clear solution.
Purity
Greater than 90% as determined by SDS-PAGE.
Usage
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

Vascular Endothelial Growth Factor Human Recombinant produced in E.Coli is a non-glycosylated, polypeptide chain (aa 207-371) containing a total of 185 amino acids and having a molecular mass of 21.3 kDa (corresponding to Isoform L-VEGF165 UniProt acc#P15692-11).
The VEGF is fused to a 20 a.a His-tag at N-terminus and is purified by proprietary chromatographic techniques.

Product Specs

Introduction
Vascular endothelial growth factor (VEGF) is a crucial signaling protein for vasculogenesis and angiogenesis. While primarily known for its effects on vascular endothelial cells, VEGF also impacts other cell types like monocytes, macrophages, neurons, cancer cells, and kidney epithelial cells. VEGF's roles include increasing vascular permeability, stimulating angiogenesis and vasculogenesis, promoting endothelial cell growth and migration, and inhibiting apoptosis. In vitro studies demonstrate its ability to induce endothelial cell mitogenesis and migration. VEGF also acts as a vasodilator, enhancing microvascular permeability, and was initially termed vascular permeability factor. Elevated VEGF levels are associated with POEMS syndrome (Crow-Fukase syndrome). Mutations in the VEGF gene are linked to both proliferative and nonproliferative diabetic retinopathy.
Description
Recombinant Human Vascular Endothelial Growth Factor, produced in E. coli, is a non-glycosylated polypeptide chain (amino acids 207-371) comprising 185 amino acids. It has a molecular weight of 21.3 kDa, corresponding to Isoform L-VEGF165 (UniProt accession number P15692-11). This VEGF protein includes a 20 amino acid His-tag at the N-terminus and is purified using proprietary chromatographic techniques.
Physical Appearance
A clear, sterile-filtered solution.
Formulation
VEGF His (0.5mg/ml) is supplied in a buffer containing 20mM Tris-HCl (pH 8.5), 50% glycerol, 5mM DTT, 200mM NaCl, and 2mM EDTA.
Stability
For short-term storage (2-4 weeks), store the entire vial at 4°C. For extended storage, freeze at -20°C. Adding a carrier protein (0.1% HSA or BSA) is recommended for long-term storage. Avoid repeated freeze-thaw cycles.
Purity
Purity is greater than 90% as determined by SDS-PAGE analysis.
Synonyms
Vascular endothelial growth factor A, VEGF-A, Vascular permeability factor, VPF, VEGF, MGC70609.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH APMAEGGGQN HHEVVKFMDV YQRSYCHPIE TLVDIFQEYP DEIEYIFKPS CVPLMRCGGC CNDEGLECVP TEESNITMQI MRIKPHQGQH IGEMSFLQHN KCECRPKKDR ARQENPCGPC SERRKHLFVQ DPQTCKCSCK NTDSRCKARQ LELNERTCRC DKPRR.

Product Science Overview

Introduction

Vascular Endothelial Growth Factor (VEGF) is a signal protein that stimulates the formation of blood vessels. It plays a crucial role in both physiological and pathological angiogenesis, which is the growth of new blood vessels from pre-existing ones. VEGF is a key player in processes such as embryonic development, wound healing, and the formation of new blood vessels in tumors.

Structure and Function

VEGF belongs to the PDGF (platelet-derived growth factor)/VEGF family and is secreted by various cell types. The human VEGF gene is located on chromosome 6p21.1 and encodes a dimeric glycoprotein consisting of two antiparallel monomers. There are several isoforms of VEGF, with VEGF165 being one of the most studied due to its potent angiogenic properties .

VEGF mediates increased vascular permeability, induces vasculogenesis (the formation of new blood vessels during embryonic development), promotes endothelial cell proliferation and migration, and inhibits apoptosis (programmed cell death). These functions make VEGF a critical factor in both normal physiological processes and in diseases such as cancer, where it promotes tumor growth and metastasis by enhancing blood supply to the tumor .

Recombinant VEGF

Recombinant VEGF is produced using various expression systems, including bacterial (E. coli) and mammalian (HEK 293) cells. The recombinant form of VEGF, particularly VEGF165, is often fused with a His-tag (a sequence of histidine residues) at the N-terminus to facilitate purification using nickel affinity chromatography . This method allows for the production of high-purity VEGF, which is essential for research and therapeutic applications.

Applications

Recombinant VEGF is widely used in research to study angiogenesis and vascular biology. It is also employed in therapeutic applications, such as promoting wound healing and tissue regeneration. In cancer research, VEGF inhibitors are being developed as potential treatments to block the blood supply to tumors, thereby inhibiting their growth and spread .

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