VEGF Rat, His

Vascular Endothelial Growth Factor Rat Recombinant, His Tag
Cat. No.
BT8454
Source
Escherichia Coli.
Synonyms
VEGF-A, Vascular permeability factor, VPF, VEGF.
Appearance
Sterile filtered colorless solution.
Purity
Greater than 90.0% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

VEGF Rat Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 145 amino acids (206-325 a.a) and having a molecular mass of 16.7kDa.
VEGF is fused to a 25 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
Vascular endothelial growth factor (VEGF) is a key signaling protein involved in the formation of new blood vessels (vasculogenesis and angiogenesis). While primarily known for its effects on vascular endothelial cells, VEGF also influences other cell types, including those involved in the immune response (monocytes and macrophages), nervous system (neurons), and kidneys (epithelial cells), as well as cancer cells. VEGF's roles encompass promoting blood vessel formation and growth, increasing blood vessel permeability, stimulating cell migration, and inhibiting cell death (apoptosis). In laboratory settings, VEGF has been observed to stimulate the division and migration of endothelial cells. Additionally, VEGF acts as a vasodilator, widening blood vessels, and enhances the permeability of small blood vessels, leading to its initial designation as vascular permeability factor. Abnormally high levels of VEGF are associated with POEMS syndrome, also known as Crow-Fukase syndrome. Furthermore, mutations in the gene responsible for producing VEGF have been linked to diabetic retinopathy, a condition affecting the eyes.
Description
Recombinant Rat VEGF, expressed in E. coli, is a single-chain polypeptide that lacks glycosylation. It comprises 145 amino acids (spanning positions 206 to 325) and has a molecular weight of 16.7 kDa. The protein includes a 25 amino acid His-tag at its N-terminus to facilitate purification, which is achieved through specialized chromatographic methods.
Physical Appearance
A clear and colorless solution that has been sterilized by filtration.
Formulation
The VEGF protein is supplied in a solution at a concentration of 0.25 mg/ml. The solution also contains phosphate-buffered saline (PBS) at a pH of 7.4 and glycerol at a concentration of 50%.
Stability
For short-term storage (up to 2-4 weeks), the VEGF protein should be stored at 4°C. For extended storage, it is recommended to freeze the protein at -20°C. Adding a carrier protein like HSA or BSA (0.1%) is advisable for long-term storage. To maintain protein integrity, avoid repeated cycles of freezing and thawing.
Purity
The purity of the VEGF protein is greater than 90%, as determined by SDS-PAGE analysis.
Synonyms
VEGF-A, Vascular permeability factor, VPF, VEGF.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSHMAPTTE GEQKAHEVVK FMDVYQRSYC RPIETLVDIF QEYPDEIEYI FKPSCVPLMR CAGCCNDEAL ECVPTSESNV TMQIMRIKPH QSQHIGEMSF LQHSRCECRP KKDRTKPEKC DKPRR

Product Science Overview

Introduction

Vascular Endothelial Growth Factor (VEGF) is a signal protein that plays a crucial role in the formation of blood vessels. It is part of the platelet-derived growth factor family and is involved in both vasculogenesis (the formation of the embryonic circulatory system) and angiogenesis (the growth of blood vessels from pre-existing vasculature) . VEGF is essential for restoring oxygen supply to tissues when blood circulation is inadequate, such as in hypoxic conditions .

VEGF in Rats

The rat recombinant VEGF, specifically with a His tag, is a version of this protein that has been engineered for research purposes. This recombinant protein is produced in insect cells and contains 129 amino acid residues. It is fused to a His-tag (6x His) at the C-terminal end, which facilitates its purification and detection . Due to glycosylation, VEGF-C migrates as an 18-24 kDa protein in SDS-PAGE under reducing conditions .

Functions and Applications

VEGF is a glycosylated mitogen that specifically acts on endothelial cells. It has various effects, including:

  • Mediating increased vascular permeability
  • Inducing angiogenesis and vasculogenesis
  • Promoting endothelial cell growth
  • Encouraging cell migration
  • Inhibiting apoptosis

These properties make VEGF a critical factor in both normal physiological processes and pathological conditions. For instance, it is involved in creating new blood vessels during embryonic development, after injury, and in muscles following exercise . Additionally, VEGF plays a role in diseases such as cancer, where it can contribute to tumor growth and metastasis by promoting blood vessel formation .

Research and Therapeutic Uses

The recombinant form of VEGF with a His tag is widely used in research to study its functions and interactions. It is also used in developing therapeutic strategies for diseases involving abnormal blood vessel growth. For example, drugs that inhibit VEGF, such as bevacizumab and ranibizumab, are used to treat cancers and retinal diseases .

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THE BIOTEK
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