VEGF Human, CHO

Vascular Endothelial Growth Factor Human Recombinant, CHO
Cat. No.
BT7407
Source
Chinese Hamster Ovarian Cell.
Synonyms
Vascular endothelial growth factor A, VEGF-A, Vascular permeability factor, VPF, VEGF, MGC70609.
Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Purity
Greater than 97.0% as determined by SDS-PAGE.
Usage
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

Vascular Endothelial Growth Factor Human Recombinant produced in CHO cells is a double, glycosylated, polypeptide chain containing 165 amino acids and migrates as 44 kDa in SDS-PAGE under non-reducing conditions.
The VEGF is purified by proprietary chromatographic techniques.

Product Specs

Introduction
Vascular endothelial growth factor (VEGF) is a key signaling protein in vasculogenesis and angiogenesis. Primarily known for its effects on vascular endothelial cells, VEGF also influences other cell types, including monocytes/macrophages, neurons, cancer cells, and kidney epithelial cells. Its actions include increasing vascular permeability, promoting angiogenesis and vasculogenesis, stimulating endothelial cell growth and migration, and inhibiting apoptosis. In vitro studies demonstrate its ability to induce endothelial cell mitogenesis and migration. VEGF also acts as a vasodilator, enhances microvascular permeability, and was initially known as vascular permeability factor. Elevated VEGF levels are associated with POEMS syndrome (Crow-Fukase syndrome). Mutations in the VEGF gene are linked to proliferative and nonproliferative diabetic retinopathy.
Description
Recombinant Human Vascular Endothelial Growth Factor, produced in CHO cells, is a glycosylated polypeptide chain dimer comprising 165 amino acids per monomer. It exhibits a molecular weight of approximately 44 kDa under non-reducing conditions in SDS-PAGE. The purification process utilizes proprietary chromatographic techniques.
Physical Appearance
Sterile, white lyophilized powder.
Formulation
The protein is lyophilized from a phosphate-buffered saline solution at pH 7.4.
Solubility
To reconstitute the lyophilized Vascular Endothelial Growth Factor Human, it is recommended to dissolve it in sterile 18 MΩ-cm H2O at a concentration of at least 100 µg/ml. The reconstituted solution can then be further diluted with other aqueous solutions.
Stability
Lyophilized Vascular Endothelial Growth Factor Human remains stable at room temperature for up to 3 weeks. However, for extended storage, it is recommended to store it desiccated at a temperature below -18°C. After reconstitution, the VEGF Human Recombinant should be stored at 4°C for 2-7 days. For long-term storage, consider adding a carrier protein (0.1% HSA or BSA) and store below -18°C. Avoid repeated freeze-thaw cycles.
Purity
Purity exceeds 97.0% as assessed by SDS-PAGE.
Biological Activity
The protein's biological activity, determined through its effect on HUVEC cells, exhibits an ED50 value ranging from 2 to 6 ng/ml.
Synonyms
Vascular endothelial growth factor A, VEGF-A, Vascular permeability factor, VPF, VEGF, MGC70609.
Source
Chinese Hamster Ovarian Cell.

Product Science Overview

Introduction

Vascular Endothelial Growth Factor (VEGF) is a sub-family of growth factors, specifically the platelet-derived growth factor family of cystine-knot growth factors . VEGF plays a crucial role in both vasculogenesis (the formation of the embryonic circulatory system) and angiogenesis (the growth of blood vessels from pre-existing vasculature) . It is a key regulator of angiogenesis and is central to processes such as tumor growth and metastatic dissemination .

Human Recombinant VEGF

Human recombinant VEGF, particularly VEGF165, is a bioactive form that has been extensively studied for its role in promoting angiogenesis and vascular permeability . The recombinant form is produced using various expression systems, including Chinese Hamster Ovary (CHO) cells, which are commonly used for the production of therapeutic proteins due to their ability to perform post-translational modifications similar to those in humans .

Preparation Methods

The production of human recombinant VEGF involves several steps:

  1. Cloning and Expression: The gene encoding VEGF is cloned into an expression vector, which is then introduced into CHO cells . These cells are cultured under specific conditions to express the VEGF protein.
  2. Purification: The expressed VEGF protein is purified using techniques such as affinity chromatography . This step ensures that the protein is isolated in a highly pure form.
  3. Characterization: The purified protein is characterized to confirm its identity, purity, and biological activity .
Chemical Reactions and Analysis

VEGF mediates its effects by binding to specific receptors on the surface of endothelial cells, primarily VEGFR-1 and VEGFR-2 . This binding activates a cascade of signaling pathways that promote endothelial cell proliferation, migration, and new blood vessel formation . The biological activity of recombinant VEGF can be assessed using various assays, such as the Chicken chorioallantoic membrane assay and wound-healing migration assays .

Applications

Recombinant VEGF has significant therapeutic potential. It is used in research to study angiogenesis and in clinical settings to promote wound healing and treat conditions such as ischemic heart disease and peripheral artery disease . Additionally, VEGF inhibitors are used in cancer therapy to inhibit tumor growth by blocking angiogenesis .

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