VEGF Mouse, Sf9

Vascular Endothelial Growth Factor Mouse Recombinant, Sf9
Cat. No.
BT8124
Source
Baculovirus Sf9 cells.
Synonyms
Vascular endothelial growth factor A, VEGF-A, Vascular permeability factor, VPF, VEGF, MGC70609.
Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Purity
Greater than 95.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
Usage
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

Vascular Endothelial Growth Factor Mouse Recombinant produced in Sf9 insect cells is a double, glycosylated, polypeptide chain containing 164 amino acids and having a molecular mass of 48 kDa.
The VEGF is purified by proprietary chromatographic techniques.

Product Specs

Introduction
Vascular endothelial growth factor (VEGF) is a key signaling protein involved in the formation of new blood vessels (vasculogenesis and angiogenesis). While primarily known for its effects on vascular endothelial cells, VEGF also influences other cell types, including monocytes/macrophages, neurons, cancer cells, and kidney epithelial cells. VEGF promotes blood vessel formation, increases blood vessel permeability, stimulates endothelial cell growth and migration, and prevents cell death. In laboratory settings, VEGF has been shown to enhance the growth and movement of endothelial cells. Additionally, VEGF acts as a vasodilator, widening blood vessels, and was initially named vascular permeability factor due to its ability to increase permeability in small blood vessels. Elevated VEGF levels are associated with POEMS syndrome, also known as Crow-Fukase syndrome. Genetic mutations in the VEGF gene have been linked to both proliferative and nonproliferative diabetic retinopathy.
Description
Recombinant Mouse Vascular Endothelial Growth Factor, produced in Sf9 insect cells, is a glycosylated polypeptide chain dimer composed of 164 amino acids, resulting in a molecular weight of 48 kDa. The purification process of VEGF involves proprietary chromatographic techniques.
Physical Appearance
Sterile Filtered White lyophilized powder.
Formulation
The protein solution was lyophilized at a concentration of 1mg/ml without any additional ingredients.
Solubility
To reconstitute the lyophilized Vascular Endothelial Growth Factor-Sf9, it is recommended to dissolve it in sterile 18MΩ-cm H2O to a concentration of at least 100µg/ml. This solution can then be diluted further into other aqueous solutions as needed.
Stability
Lyophilized Vascular Endothelial Growth Factor Sf9 remains stable at room temperature for up to 3 weeks. However, for long-term storage, it is recommended to store it desiccated at a temperature below -18°C. Once reconstituted, VEGF-Sf9 should be stored at 4°C for a period of 2-7 days. For future use, it should be stored at -18°C. To ensure optimal stability during long-term storage, the addition of a carrier protein (0.1% HSA or BSA) is recommended. Avoid repeated freeze-thaw cycles.
Purity
The purity is determined to be greater than 95.0% through the following analyses: (a) Reverse-Phase High-Performance Liquid Chromatography (RP-HPLC) and (b) Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis (SDS-PAGE).
Biological Activity
The ED50 range, which represents the concentration of VEGF required to achieve 50% of the maximum biological response, is determined to be within 1-2 ng/ml. This determination is based on the dose-dependent proliferation of human umbilical vein endothelial cells (HUVEC) measured through 3H-thymidine uptake. This corresponds to a specific activity of 1x10^6 Units/mg.
Synonyms
Vascular endothelial growth factor A, VEGF-A, Vascular permeability factor, VPF, VEGF, MGC70609.
Source
Baculovirus Sf9 cells.

Product Science Overview

Introduction

Vascular Endothelial Growth Factor (VEGF) is a critical signaling protein involved in the processes of vasculogenesis and angiogenesis. It primarily affects cells of the vascular endothelium, promoting the growth of new blood vessels. VEGF is also known for its role in increasing vascular permeability and is sometimes referred to as Vascular Permeability Factor (VPF).

Structure and Function

VEGF is a glycosylated protein that exists in several isoforms due to alternative splicing of its mRNA. The mouse recombinant VEGF produced in Sf9 insect cells is a double, glycosylated polypeptide chain containing 164 amino acids and has a molecular mass of approximately 48 kDa . This recombinant form is produced using a baculovirus expression system in Sf9 insect cells, which allows for proper folding and post-translational modifications.

Biological Activity

VEGF plays a pivotal role in both physiological and pathological angiogenesis. It mediates increased vascular permeability, induces angiogenesis and vasculogenesis, promotes endothelial cell growth, and inhibits apoptosis. In vitro studies have shown that VEGF stimulates endothelial cell mitogenesis and cell migration . It is also a vasodilator and increases microvascular permeability.

Applications

Recombinant VEGF is widely used in research to study its effects on various cell types, including endothelial cells, neurons, cancer cells, and kidney epithelial cells. It is also used in assays to investigate the mechanisms of angiogenesis and to screen for potential inhibitors of VEGF signaling pathways .

Production and Purification

The mouse recombinant VEGF produced in Sf9 cells is purified using proprietary chromatographic techniques to achieve a purity greater than 95% . The protein is typically lyophilized and can be reconstituted in sterile water for experimental use. It is recommended to store the lyophilized protein desiccated below -18°C for long-term stability .

Clinical Relevance

Elevated levels of VEGF are associated with various pathological conditions, including proliferative and non-proliferative diabetic retinopathy, and POEMS syndrome (also known as Crow-Fukase syndrome). Understanding the role of VEGF in these conditions can help in developing targeted therapies to modulate its activity .

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