VEGF Human, HEK

Vascular Endothelial Growth Factor Human Recombinant, HEK
Cat. No.
BT7517
Source
HEK293 (Human Embryonic Kidney cell line).
Synonyms
Vascular endothelial growth factor A, VEGF-A, Vascular permeability factor, VPF, VEGF, MGC70609.
Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Purity
Greater than 95.0% as determined by SDS-PAGE.
Usage
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

Vascular Endothelial Growth Factor Human Recombinant produced in HEK293 cells is a double, glycosylated, polypeptide chain containing 165 amino acids (27-191) and having a molecular mass of 40 kDa. The VEGF is purified by proprietary chromatographic techniques.

Product Specs

Introduction
Vascular endothelial growth factor (VEGF) is a crucial signaling protein for vasculogenesis and angiogenesis, primarily affecting vascular endothelial cells. However, it also impacts other cell types like monocytes, macrophages, neurons, cancer cells, and kidney epithelial cells. VEGF enhances vascular permeability, stimulates angiogenesis and vasculogenesis, promotes endothelial cell growth and migration, and inhibits apoptosis. In vitro studies demonstrate its ability to stimulate endothelial cell mitogenesis and migration. VEGF also acts as a vasodilator, increasing microvascular permeability, hence its initial name, vascular permeability factor. Elevated VEGF levels are linked to POEMS syndrome (Crow-Fukase syndrome). Gene mutations associated with VEGF are implicated in both proliferative and nonproliferative diabetic retinopathy.
Description
Recombinant Human Vascular Endothelial Growth Factor, produced in HEK293 cells, is a glycosylated polypeptide chain dimer. It comprises 165 amino acids (27-191) and has a molecular weight of 40 kDa. The purification process involves proprietary chromatographic techniques.
Physical Appearance
Sterile, white lyophilized powder.
Formulation
The protein was lyophilized from a 0.2µM filtered solution containing 20mM PB (phosphate buffer), 150mM NaCl (sodium chloride), at pH 7.2.
Solubility
Reconstitute the lyophilized Vascular Endothelial Growth Factor-HEK in sterile 18 MΩ-cm H2O to a concentration of at least 100 µg/ml. This solution can be further diluted with other aqueous solutions.
Stability
Lyophilized Vascular Endothelial Growth Factor HEK remains stable at room temperature for up to 3 weeks. However, for long-term storage, it is recommended to store desiccated below -18°C. Once reconstituted, VEGF HEK should be stored at 4°C for 2-7 days. For future use, store below -18°C. Adding a carrier protein (0.1% HSA or BSA) is recommended for extended storage. Avoid repeated freeze-thaw cycles.
Purity
Purity exceeds 95.0% as determined by SDS-PAGE.
Biological Activity
The biological activity is determined by the dose-dependent stimulation of human umbilical vein endothelial cell (HUVEC) proliferation. This is assessed within a concentration range of 4.0 ng/ml, corresponding to a specific activity of 2.5 x 10⁵ Units/mg.
Synonyms
Vascular endothelial growth factor A, VEGF-A, Vascular permeability factor, VPF, VEGF, MGC70609.
Source
HEK293 (Human Embryonic Kidney cell line).
Amino Acid Sequence
APMAEGGGQN HHEVVKFMDV YQRSYCHPIE TLVDIFQEYP DEIEYIFKPS CVPLMRCGGC CNDEGLECVP TEESNITMQI MRIKPHQGQH IGEMSFLQHN KCECRPKKDR ARQENPCGPC SERRKHLFVQ DPQTCKCSCK NTDSRCKARQ LELNERTCRC DKPRR.

Product Science Overview

Introduction

Vascular Endothelial Growth Factor (VEGF) is a potent growth factor that plays a crucial role in angiogenesis, the formation of new blood vessels from pre-existing ones. This protein is essential for various physiological processes, including wound healing, embryonic development, and the formation of new blood vessels in response to tissue ischemia. VEGF is also implicated in pathological conditions such as cancer, where it promotes tumor growth by enhancing blood supply to the tumor cells.

Structure and Isoforms

VEGF is a glycosylated protein that exists in several isoforms due to alternative splicing of its mRNA. The most studied isoform is VEGF165, which consists of 165 amino acids. This isoform is a dimeric glycoprotein, meaning it is composed of two identical subunits linked together. The molecular weight of VEGF165 is approximately 45 kDa when glycosylated .

Expression in HEK 293 Cells

Human Embryonic Kidney (HEK) 293 cells are commonly used for the recombinant expression of VEGF. These cells are advantageous because they provide authentic human glycosylation patterns, which are crucial for the stability and biological activity of the protein. The recombinant VEGF produced in HEK 293 cells is often referred to as VEGF (Human Recombinant, HEK) .

Biological Functions

VEGF primarily acts on endothelial cells, the cells lining the interior surface of blood vessels. It binds to specific receptors on these cells, initiating a cascade of signaling events that lead to:

  • Proliferation: VEGF stimulates the division and multiplication of endothelial cells.
  • Migration: It promotes the movement of endothelial cells to the site where new blood vessels are needed.
  • Permeability: VEGF increases the permeability of blood vessels, allowing essential nutrients and oxygen to reach tissues more effectively .
Clinical Significance

VEGF is a critical factor in both physiological and pathological angiogenesis. In cancer, VEGF is often overexpressed, leading to the formation of new blood vessels that supply the tumor with nutrients and oxygen, facilitating its growth and metastasis. Anti-VEGF therapies, such as monoclonal antibodies and small molecule inhibitors, have been developed to target this pathway and are used in the treatment of various cancers .

In addition to its role in cancer, VEGF is also involved in other diseases characterized by abnormal blood vessel growth, such as age-related macular degeneration and diabetic retinopathy. Therapeutic strategies targeting VEGF are being explored for these conditions as well .

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