VEGF (121a.a.) Human, HEK

Vascular Endothelial Growth Factor (121) Human Recombinant, HEK
Cat. No.
BT6314
Source
HEK.
Synonyms
Vascular endothelial growth factor A, VEGF-A, Vascular permeability factor, VPF, VEGF, MGC70609.
Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Purity
Greater than 95% as obsereved by SDS-PAGE.
Usage
THE BioTeks products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

Recombinant Human VEGF 121 amino acids, produced in HEK cells is a glycosylated 37kDa homodimer and 50kDa homotrimer.
The VEGF is purified by proprietary chromatographic techniques.

Product Specs

Introduction
Vascular endothelial growth factor (VEGF) is a key signaling protein that plays a crucial role in both vasculogenesis (formation of new blood vessels) and angiogenesis (growth of new blood vessels from existing ones). VEGF's primary function is to promote the growth and maintenance of blood vessels. Its effects are most notable on vascular endothelial cells, which line the interior of blood vessels. However, VEGF also impacts other cell types, including cells involved in the immune response (monocytes and macrophages), nerve cells (neurons), tumor cells, and kidney epithelial cells. VEGF's diverse functions encompass: enhancing vascular permeability, stimulating angiogenesis and vasculogenesis, promoting endothelial cell growth and migration, and inhibiting programmed cell death (apoptosis). In laboratory settings, VEGF has been shown to induce the proliferation and movement of endothelial cells. Initially known as vascular permeability factor, VEGF also acts as a vasodilator, widening blood vessels, and increasing the permeability of small blood vessels (microvasculature). Elevated levels of VEGF have been linked to POEMS syndrome, also known as Crow-Fukase syndrome. Genetic mutations in the VEGF gene are associated with both proliferative and nonproliferative diabetic retinopathy.
Description
Recombinant Human VEGF, encompassing 121 amino acids and produced in HEK cells, is a glycosylated protein. It exists in two forms: a homodimer with a molecular weight of 37kDa and a homotrimer with a molecular weight of 50kDa. The VEGF protein undergoes a purification process using specialized chromatographic techniques.
Physical Appearance
Sterile Filtered White lyophilized powder.
Formulation
The VEGF protein was lyophilized from a 0.2µm filtered solution containing 1xPBS.
Solubility
For reconstitution of the lyophilized VEGF, it is recommended to use sterile PBS containing 0.1% endotoxin-free recombinant HSA.
Stability
Lyophilized VEGF remains stable at room temperature for up to 3 weeks. However, for extended storage, it is recommended to store it in a dry environment below -18°C. Once reconstituted, VEGF can be stored at 4°C for 2-7 days. For long-term storage after reconstitution, it should be kept at -18°C. The addition of a carrier protein (0.1% HSA or BSA) is recommended for long-term storage. Avoid repeated freeze-thaw cycles to maintain protein stability.
Purity
The purity of the protein is greater than 95% as determined by SDS-PAGE analysis.
Biological Activity
The biological activity of the VEGF protein was assessed by its ability to stimulate the proliferation of HUVEC cells (Human Umbilical Vein Endothelial Cells) in a dose-dependent manner. The ED50, which represents the concentration of VEGF required to achieve 50% of the maximal proliferative response, was determined to be 3ng/ml.
Synonyms
Vascular endothelial growth factor A, VEGF-A, Vascular permeability factor, VPF, VEGF, MGC70609.
Source
HEK.

Product Science Overview

Introduction

Vascular Endothelial Growth Factor 121 (VEGF121) is a significant isoform of the Vascular Endothelial Growth Factor A (VEGFA) family. VEGF121 is a potent angiogenic factor that plays a crucial role in the formation of blood vessels, a process known as angiogenesis. This article delves into the background, structure, function, and applications of VEGF121, particularly focusing on its recombinant form expressed in Human Embryonic Kidney (HEK) 293 cells.

Structure and Isoforms

VEGF121 is one of the several isoforms of VEGFA, which also includes VEGF165 and others. These isoforms arise due to alternative splicing of the VEGFA gene. VEGF121 is characterized by its smaller size compared to other isoforms, with a molecular mass of approximately 37 kDa as a homodimer and 50 kDa as a homotrimer . The recombinant form of VEGF121 expressed in HEK 293 cells is glycosylated, which contributes to its stability and functionality in various biological applications .

Biological Function

VEGF121 is a critical player in angiogenesis, promoting the proliferation, migration, and survival of endothelial cells. It binds to specific receptors on the surface of endothelial cells, primarily VEGFR1 and VEGFR2, initiating a cascade of signaling pathways that lead to the formation of new blood vessels . This process is vital for normal physiological functions such as wound healing and the menstrual cycle, as well as pathological conditions like tumor growth and metastasis .

Expression and Production

The recombinant form of VEGF121 is produced in HEK 293 cells, which are human embryonic kidney cells widely used in biotechnology for protein expression. The use of HEK 293 cells ensures that the recombinant protein undergoes proper post-translational modifications, including glycosylation, which is essential for its stability and activity . The production process involves the insertion of the VEGFA gene into the HEK 293 cells, followed by the purification of the expressed protein to achieve high purity levels suitable for research and therapeutic applications .

Applications

VEGF121 has numerous applications in both research and clinical settings. In research, it is used to study angiogenesis and related signaling pathways. It is also employed in cell culture systems to promote the growth and maintenance of endothelial cells . Clinically, VEGF121 and its analogs are explored for their potential in treating conditions that require enhanced blood vessel formation, such as ischemic diseases and wound healing . Additionally, VEGF121 is being investigated for its role in cancer therapy, as inhibiting its activity can potentially reduce tumor growth and metastasis .

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