VEGF Human, Baculovirus

Vascular Endothelial Growth Factor Human Recombinant, Baculovirus
Cat. No.
BT7335
Source
Sf9, Baculovirus cells.
Synonyms
Vascular Endothelial Growth Factor A, VEGF, Vascular Permeability Factor, MVCD1, VPF, Vascular Endothelial Growth Factor, VEGF-A, Vascular endothelial growth factor A.
Appearance
Sterile filtered colorless solution.
Purity
Greater than 85.0% as determined by SDS-PAGE.
Usage

THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

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Description

VEGF produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 171 amino acids (27-191 a.a.) and having a molecular mass of 19.9 kDa.

VEGF is expressed with a 6 amino acid His tag at C-Terminus and purified by proprietary chromatographic techniques.

Product Specs

Introduction
Vascular endothelial growth factor (VEGF) is a crucial signaling protein for vasculogenesis and angiogenesis. While primarily studied for its effects on vascular endothelial cells, VEGF also influences other cell types like monocytes/macrophages, neurons, cancer cells, and kidney epithelial cells. VEGF contributes to increased vascular permeability, angiogenesis, vasculogenesis, endothelial cell growth, cell migration, and apoptosis inhibition. In vitro studies show VEGF stimulates endothelial cell mitogenesis and migration. Acting as a vasodilator, VEGF increases microvascular permeability, leading to its initial name as vascular permeability factor. Notably, elevated VEGF levels are linked to POEMS syndrome (Crow-Fukase syndrome). Furthermore, mutations in the VEGF gene are associated with both proliferative and nonproliferative diabetic retinopathy.
Description
Produced in Sf9 Baculovirus cells, this recombinant VEGF protein is a single, glycosylated polypeptide chain. It consists of 171 amino acids (specifically, amino acids 27-191), resulting in a molecular mass of 19.9 kDa. For purification and detection purposes, a 6 amino acid His tag is present at the C-terminus. The protein is purified using proprietary chromatographic techniques.
Physical Appearance
The product is a sterile, colorless solution that has been filtered for sterility.
Formulation
The VEGF protein solution is provided at a concentration of 0.25mg/ml. The solution is formulated in a phosphate-buffered saline (PBS) at pH 7.4 and supplemented with 30% glycerol, 1mM DTT (dithiothreitol), and 0.1mM PMSF (phenylmethylsulfonyl fluoride).
Stability
For short-term storage (up to 2-4 weeks), the VEGF protein should be stored at 4°C. For long-term storage, it is recommended to store the protein frozen at -20°C. To further enhance stability during long-term storage, consider adding a carrier protein such as HSA (human serum albumin) or BSA (bovine serum albumin) to a final concentration of 0.1%. To maintain protein integrity, avoid repeated freeze-thaw cycles.
Purity
The purity of the VEGF protein is greater than 85%, as determined by SDS-PAGE analysis.
Synonyms
Vascular Endothelial Growth Factor A, VEGF, Vascular Permeability Factor, MVCD1, VPF, Vascular Endothelial Growth Factor, VEGF-A, Vascular endothelial growth factor A.
Source
Sf9, Baculovirus cells.
Amino Acid Sequence

APMAEGGGQN HHEVVKFMDV YQRSYCHPIE TLVDIFQEYP DEIEYIFKPS CVPLMRCGGC CNDEGLECVP TEESNITMQI MRIKPHQGQH IGEMSFLQHN KCECRPKKDR ARQENPCGPC SERRKHLFVQ DPQTCKCSCK NTDSRCKARQ LELNERTCRC DKPRRHHHHH H.

Product Science Overview

Introduction

Vascular Endothelial Growth Factor (VEGF) is a critical signaling protein involved in the formation of blood vessels, a process known as angiogenesis. VEGF plays a pivotal role in both physiological and pathological conditions, including embryonic development, wound healing, and tumor growth .

VEGF Family and Isoforms

The VEGF family consists of several proteins, including VEGF-A, VEGF-B, VEGF-C, VEGF-D, and Placental Growth Factor (PlGF). Among these, VEGF-A is the most studied and is often referred to simply as VEGF. VEGF-A binds to two primary receptors, VEGFR-1 (Flt-1) and VEGFR-2 (KDR/Flk-1), which mediate its angiogenic effects .

Human Recombinant VEGF

Human recombinant VEGF is produced using various expression systems, including Escherichia coli, yeast, and baculovirus-infected insect cells. The baculovirus expression system is particularly advantageous for producing complex proteins with post-translational modifications similar to those in mammalian cells .

Baculovirus Expression System

The baculovirus expression system utilizes insect cells, such as Sf9 or Sf21, to produce recombinant proteins. This system is highly efficient and capable of producing large quantities of protein. The recombinant human VEGF produced in this system is often tagged with a histidine tag to facilitate purification using nickel affinity chromatography .

Applications and Clinical Significance

VEGF is a key player in promoting angiogenesis and vascular permeability. It is extensively studied for its role in cancer, where it promotes tumor growth and metastasis by enhancing blood supply to the tumor. Anti-VEGF therapies, such as monoclonal antibodies and small molecule inhibitors, are used in the treatment of various cancers and other diseases characterized by abnormal angiogenesis .

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