VEGF Mouse, His

Vascular Endothelial Growth Factor Mouse Recombinant, His Tag
Cat. No.
BT8030
Source
Escherichia Coli.
Synonyms
Vascular endothelial growth factor A, VEGF-A, Vascular permeability factor, VPF, VEGF, Vegf120, Vegf164, Vegf188, Vegfa.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 90.0% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Shipped with Ice Packs
In Stock
Quick Inquiry

Description

VEGF Mouse Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 141 amino acids (205-324 a.a.) and having a total molecular mass of 16.3kDa.
Mouse VEGF is fused to 20 amino acid His Tag at N-terminus and purified by proprietary chromatographic techniques.

Product Specs

Introduction
Vascular endothelial growth factor (VEGF) is a crucial signaling protein involved in the formation of new blood vessels (vasculogenesis and angiogenesis). While primarily known for its effects on vascular endothelial cells, VEGF also influences other cell types such as immune cells, neurons, cancer cells, and kidney epithelial cells. VEGF promotes blood vessel formation, growth, and permeability, stimulates cell migration, and inhibits cell death. In laboratory settings, VEGF has been observed to enhance the growth and movement of endothelial cells. VEGF also acts as a vasodilator, widening blood vessels, and was initially known as vascular permeability factor due to its ability to increase blood vessel permeability. Elevated levels of VEGF are associated with POEMS syndrome, also called Crow-Fukase syndrome. Genetic mutations in the VEGF gene have been linked to diabetic retinopathy, a condition affecting the eyes.
Description
Recombinant Mouse VEGF, produced in E. coli bacteria, is a single, non-glycosylated polypeptide chain comprising 141 amino acids (corresponding to amino acids 205-324 of the full-length protein). It has a molecular weight of 16.3 kDa. This Mouse VEGF variant is fused to a 20 amino acid Histidine (His) tag at its N-terminus to facilitate purification, which is carried out using specialized chromatographic methods.
Physical Appearance
Clear, colorless solution that has been sterilized by filtration.
Formulation
Mouse VEGF is supplied in a solution containing 20mM Tris-HCl buffer at pH 8.0 and 10% glycerol.
Stability
For optimal storage, refrigerate at 4°C if the entire vial will be used within 2-4 weeks. For longer-term storage, freeze at -20°C. To enhance stability during long-term storage, consider adding a carrier protein such as 0.1% HSA or BSA. Repeated freezing and thawing should be avoided.
Purity
Purity exceeds 90.0% as determined by SDS-PAGE analysis.
Biological Activity
Biological activity is assessed through a cell proliferation assay utilizing NIH-3T3 mouse embryonic fibroblasts. The ED50, which represents the concentration at which 50% of the maximum effect is observed, is within the range of 0.5-1.5 ng/ml.
Synonyms
Vascular endothelial growth factor A, VEGF-A, Vascular permeability factor, VPF, VEGF, Vegf120, Vegf164, Vegf188, Vegfa.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MAPTTEGEQK SHEVIKFMDV YQRSYCRPIE TLVDIFQEYP DEIEYIFKPS CVPLMRCAGC CNDEALECVP TSESNITMQI MRIKPHQSQH IGEMSFLQHS RCECRPKKDR TKPEKCDKPR R.

Product Science Overview

Introduction

Vascular Endothelial Growth Factor (VEGF) is a potent mediator of angiogenesis and vasculogenesis, playing a crucial role in both embryonic development and adult physiology. The recombinant form of VEGF, specifically the mouse variant with a His tag, is widely used in research to study its biological functions and therapeutic potential.

Structure and Expression

The recombinant mouse VEGF protein with a His tag is typically expressed in HEK 293 cells, ensuring high purity and biological activity. The His tag, a sequence of histidine residues, facilitates the purification of the protein through affinity chromatography. This recombinant protein is often produced with a purity greater than 95% and an endotoxin level of less than 1 EU/µg, making it suitable for various experimental applications .

Biological Functions

VEGF is essential for the proliferation, migration, and survival of endothelial cells. It binds to specific receptors on the surface of these cells, such as VEGFR-2 (Flk-1/KDR) and VEGFR-3 (Flt4), triggering a cascade of signaling events that promote angiogenesis. In addition to its role in blood vessel formation, VEGF also increases vascular permeability, which is critical during wound healing and in pathological conditions like tumor growth .

Applications in Research

Recombinant mouse VEGF with a His tag is used in a variety of research applications, including:

  • Angiogenesis Studies: Investigating the mechanisms of blood vessel formation and identifying potential therapeutic targets for diseases characterized by abnormal angiogenesis, such as cancer and diabetic retinopathy.
  • Drug Screening: Evaluating the efficacy of anti-angiogenic drugs that target VEGF signaling pathways.
  • Cell Culture: Supplementing cell culture media to study the effects of VEGF on endothelial cell behavior and function.

Quick Inquiry

Personal Email Detected
Please use an institutional or corporate email address for inquiries. Personal email accounts ( such as Gmail, Yahoo, and Outlook) are not accepted. *

Category

Service

Related Products

VEGF (121a.a.) Human, HEK
Vascular Endothelial Growth Factor (121) Human Recombinant, HEK
Cat. No.
BT6314
Source
HEK.
VEGF Human
Vascular Endothelial Growth Factor Human Recombinant
Cat. No.
BT7088
Source
Escherichia Coli.
VEGF Human, Yeast
Vascular Endothelial Growth Factor Human Recombinant, Yeast
Cat. No.
BT7754
Source
Pichia Pastoris.
VEGF Antibody
Vascular Endothelial Cell Growth Factor, Mouse Anti-Human
Cat. No.
BT10401
Source
THE BIOTEK
THE BioTek is a global biotech company offering highly purified proteins for research in cancer, apoptosis, development, endocrinology, immunology, neuroscience, proteases, and stem cells.
  • Contact
  • Address: 1925 E Maple Ave, El Segundo, CA 90245 United States
  • Phone : +1 201-285-7826
  • Email : info@thebiotek.com
  • Hours : Mon.-Fri. 9:00 AM - 5:00 PM
© Copyright 2024 Thebiotek. All Rights Reserved.