VEGF Human (121 a.a.), His

Vascular Endothelial Growth Factor-121 Human Recombinant, His Tag
Cat. No.
BT7236
Source
Escherichia Coli.
Synonyms
Vascular endothelial growth factor A, VEGF-A, Vascular permeability factor, VPF, VEGF, MGC70609.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 90.0% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

Vascular Endothelial Growth Factor-121 Human Recombinant produced in E.Coli is a double, non-glycosylated, polypeptide chain (aa 207-327) containing a total of 142 amino acids and having a molecular mass of 16.3 kDa.
The VEGF-121 is fused to a 20 amino acid His tag at N-terminus and purified by proprietary chromatographic techniques.

Product Specs

Introduction
Vascular endothelial growth factor (VEGF) is a crucial signaling protein for vasculogenesis and angiogenesis. While primarily studied for its effects on vascular endothelial cells, VEGF also influences other cell types like monocytes/macrophages, neurons, cancer cells, and kidney epithelial cells. VEGF promotes vascular permeability, angiogenesis, vasculogenesis, endothelial cell growth, and cell migration, while also inhibiting apoptosis. In vitro studies demonstrate its ability to stimulate endothelial cell mitogenesis and migration. Originally known as vascular permeability factor, VEGF acts as a vasodilator and enhances microvascular permeability. Elevated VEGF levels are associated with POEMS syndrome (Crow-Fukase syndrome). Additionally, mutations in the VEGF gene are linked to proliferative and nonproliferative diabetic retinopathy.
Description
Recombinant Human Vascular Endothelial Growth Factor-121, expressed in E. coli, is a non-glycosylated polypeptide chain comprising amino acids 207-327. This 142-amino acid protein, with a molecular weight of 16.3 kDa, consists of two identical polypeptide chains. A 20-amino acid His tag is fused to the N-terminus of VEGF-121. Purification is achieved using proprietary chromatographic techniques.
Physical Appearance
Clear, colorless solution that has been sterilized by filtration.
Formulation
VEGF-121 His Tag is supplied in a buffer consisting of 20mM Tris (pH 8.0) and 10% glycerol.
Stability
For short-term storage (2-4 weeks), keep at 4°C. For extended storage, freeze at -20°C. Adding a carrier protein (0.1% HSA or BSA) is recommended for long-term storage. Avoid repeated freeze-thaw cycles.
Purity
Purity is determined to be greater than 90.0% using SDS-PAGE analysis.
Biological Activity
The ED50, determined by a cell proliferation assay using NIH-3T3 cells, is less than 4.2 ng/ml. This corresponds to a specific activity of less than 238,095.23 units/mg.
Synonyms
Vascular endothelial growth factor A, VEGF-A, Vascular permeability factor, VPF, VEGF, MGC70609.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MAPMAEGGGQ NHHEVVKFMD VYQRSYCHPI ETLVDIFQEY PDEIEYIFKP SCVPLMRCGG CCNDEGLECV PTEESNITMQ IMRIKPHQGQ HIGEMSFLQH NKCECRPKKD RARQEKCDKP RR.

Product Science Overview

Structure and Characteristics

VEGF-121 is one of the several isoforms of VEGF-A, generated through alternative splicing of the VEGF gene. It is composed of 121 amino acids and is known for its ability to stimulate the proliferation and survival of endothelial cells, which line the interior surface of blood vessels . Unlike some other VEGF isoforms, VEGF-121 lacks heparin-binding domains, which affects its interaction with the extracellular matrix and its bioavailability.

Production and Purification

Recombinant VEGF-121 is often produced in bacterial systems such as Escherichia coli (E. coli). The recombinant protein is typically tagged with a His-tag to facilitate purification through affinity chromatography. This method ensures high purity and activity of the protein, making it suitable for various research and therapeutic applications .

Biological Functions

VEGF-121 plays a crucial role in:

  • Angiogenesis: It promotes the formation of new blood vessels, which is essential for tissue growth and repair.
  • Vascular Permeability: VEGF-121 increases the permeability of blood vessels, allowing for the efficient exchange of nutrients and waste products.
  • Cell Survival and Migration: It supports the survival and migration of endothelial cells, contributing to the maintenance and repair of the vascular system .
Clinical and Research Applications

Due to its significant role in angiogenesis, VEGF-121 is extensively studied in the context of cancer research. Tumors often exploit VEGF to promote blood vessel formation, ensuring a sufficient supply of nutrients and oxygen for their growth. Inhibiting VEGF activity is a therapeutic strategy in cancer treatment, aiming to starve the tumor by cutting off its blood supply .

Additionally, VEGF-121 is used in research to study various aspects of vascular biology, including the mechanisms of blood vessel formation, the effects of hypoxia (low oxygen conditions), and the response of endothelial cells to different stimuli .

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