TNFSF14 Mouse
LIGHT Mouse Recombinant
Cat. No.
BT29148
Source
Escherichia Coli.
Synonyms
Tumor necrosis factor ligand superfamily member 14, CD258, Tnfsf14, Light.
Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Purity
Greater than 96.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Shipped with Ice Packs 
In Stock 
Description
TNFSF14 Mouse Recombinant produced in E. Coli is a single, non-glycosylated, polypeptide chain containing 168 amino acids and having a molecular mass of 18.4kDa.
Product Specs
Introduction
TNFRSF14 belongs to the TNF receptor superfamily and is a type I transmembrane protein. It is expressed in various cell types, including peripheral blood T cells, B cells, monocytes, and tissues rich in lymphoid cells. TNFRSF14 acts as a co-stimulatory factor, activating lymphoid cells and inhibiting herpesvirus infection. It also promotes T cell proliferation and induces apoptosis in various tumor cells.
Description
Recombinant TNFSF14 from mouse, produced in E. coli, is a single, non-glycosylated polypeptide chain with 168 amino acids. It has a molecular weight of 18.4kDa.
Physical Appearance
The product is supplied as a sterile, white powder that has been lyophilized (freeze-dried).
Formulation
The TNFSF14 protein was lyophilized from a 0.2µm filtered solution in PBS at a pH of 7.4.
Solubility
To reconstitute the lyophilized TNFSF14, it is recommended to dissolve it in sterile 100mM acetic acid (HAc) at a concentration of at least 100µg/ml. This solution can be further diluted into other aqueous solutions.
Stability
Lyophilized TNFSF14 is stable at room temperature for up to 3 weeks. However, it is recommended to store it desiccated at a temperature below -18°C. After reconstitution, TNFSF14 should be stored at 4°C for 2-7 days. For long-term storage, it is recommended to store it at -18°C with a carrier protein (0.1% HSA or BSA) added. Avoid repeated freeze-thaw cycles.
Purity
The purity of the TNFSF14 is greater than 96.0%, as determined by: (a) Reverse-phase high-performance liquid chromatography (RP-HPLC) and (b) Sodium dodecyl-sulfate polyacrylamide gel electrophoresis (SDS-PAGE).
Biological Activity
The ED50, determined by a cytotoxicity assay using human HT-29 cells, is less than 2µg/ml. This corresponds to a specific activity of greater than 500 IU/mg in the presence of murine anti-polyHistidine monoclonal antibody and recombinant human interferon-gamma (rHuIFN-γ).
Synonyms
Tumor necrosis factor ligand superfamily member 14, CD258, Tnfsf14, Light.
Source
Escherichia Coli.
Amino Acid Sequence
DGGKGSWEKL IQDQRSHQAN PAAHLTGANA SLIGIGGPLL WETRLGLAFL RGLTYHDGAL VTMEPGYYYV YSKVQLSGVG CPQGLANGLP ITHGLYKRTS RYPKELELLV SRRSPCGRAN SSRVWWDSSF LGGVVHLEAG EEVVVRVPGN RLVRPRDGTR SYFGAFMV.
Product Science Overview
Structure and Expression
LIGHT is a type II transmembrane protein that consists of 240 amino acids. The recombinant form of mouse LIGHT is typically produced in various expression systems, such as mouse myeloma cell lines or baculovirus-insect cells . The protein has a predicted molecular mass of approximately 21 kDa, but it may appear larger on SDS-PAGE due to glycosylation .
Receptors and Binding
LIGHT binds to and signals through two distinct TNF receptor superfamily members:
- Herpes Virus Entry Mediator (HVEM/TNFRSF14): Predominantly expressed by T cells.
- Lymphotoxin Beta Receptor (LTβR/TNFRSF3): Expressed by stromal cells and non-lymphoid hematopoietic cells .
In humans, LIGHT also binds to a soluble decoy receptor known as DcR3 (TNFRSF6B), which can modulate its function .
Biological Functions
LIGHT plays a crucial role in the immune system, including:
- T Cell Co-stimulation: LIGHT can co-stimulate T cell responses via HVEM, which is constitutively expressed in most lymphocyte subpopulations .
- Tumor Suppression: LIGHT has been shown to suppress tumor formation in vivo and induce tumor cell apoptosis by up-regulating intercellular adhesion molecule 1 (ICAM-1) and increasing lymphocyte adhesion to cancer cells .
Applications
Quick Inquiry
Personal Email Detected
Please use an institutional or corporate email address for inquiries. Personal email accounts ( such as Gmail, Yahoo, and Outlook) are not accepted. *
