TNFRSF14 Human, Sf9
HVEM-Fc Human Recombinant, Sf9
Cat. No.
BT27964
Source
Sf9, Baculovirus cells.
Synonyms
Tumor necrosis factor receptor superfamily member 14 isoform 1, TNFRSF14, ATAR, CD270, HVEA, HVEM, LIGHTR, TR2, HVEM-Fc, Sf9, Tumor necrosis factor receptor superfamily member 14, Herpes virus entry mediator A, Herpesvirus entry mediator A, HveA.
Appearance
Sterile Filtered clear solution.
Purity
Greater than 90.0% as determined by analysis by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description
TNFRSF14 Human Recombinant produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 406 amino acids (39-202) and having a molecular mass of 46.6kDa (Molecular size on SDS-PAGE will appear at approximately 40-57kDa).
TNFRSF14 is fused to a 239 amino acid IgG His-Tag at C-terminus and purified by proprietary chromatographic techniques.
TNFRSF14 is fused to a 239 amino acid IgG His-Tag at C-terminus and purified by proprietary chromatographic techniques.
Product Specs
Introduction
TNFRSF14, a member of the TNF receptor superfamily, is a type I transmembrane protein. It is expressed in various immune cells like peripheral blood T cells, B cells, monocytes and tissues rich in lymphoid cells. TNFRSF14 acts as a co-stimulatory factor for activating lymphoid cells and helps in preventing herpesvirus infection. Additionally, it promotes the proliferation of T cells and induces apoptosis in various tumor cells.
Description
Recombinant human TNFRSF14 protein, produced in Sf9 Baculovirus cells, is a single, glycosylated polypeptide chain. It consists of 406 amino acids (39-202) with a molecular weight of 46.6kDa (Appears as approximately 40-57kDa on SDS-PAGE). The TNFRSF14 protein is fused to a 239 amino acid IgG His-Tag at the C-terminus and purified using proprietary chromatographic techniques.
Physical Appearance
The product is a clear, sterile filtered solution.
Formulation
The TNFRSF14 protein solution has a concentration of 0.25mg/ml and is supplied in Phosphate Buffered Saline (pH 7.4) containing 10% glycerol.
Stability
For short-term storage (2-4 weeks), store the vial at 4°C. For extended storage, freeze at -20°C. Adding a carrier protein (0.1% HSA or BSA) is recommended for long-term storage. Avoid repeated freezing and thawing cycles.
Purity
The purity of the protein is greater than 90% as determined by SDS-PAGE analysis.
Synonyms
Tumor necrosis factor receptor superfamily member 14 isoform 1, TNFRSF14, ATAR, CD270, HVEA, HVEM, LIGHTR, TR2, HVEM-Fc, Sf9, Tumor necrosis factor receptor superfamily member 14, Herpes virus entry mediator A, Herpesvirus entry mediator A, HveA.
Source
Sf9, Baculovirus cells.
Amino Acid Sequence
ADPLPSCKED EYPVGSECCP KCSPGYRVKE ACGELTGTVC EPCPPGTYIA HLNGLSKCLQ CQMCDPAMGL RASRNCSRTE NAVCGCSPGH FCIVQDGDHC AACRAYATSS PGQRVQKGGT ESQDTLCQNC PPGTFSPNGT LEECQHQTKC SWLVTKAGAG TSSSHWVLEP KSCDKTHTCP PCPAPELLGG PSVFLFPPKP KDTLMISRTP EVTCVVVDVS HEDPEVKFNW YVDGVEVHNA KTKPREEQYN STYRVVSVLT VLHQDWLNGK EYKCKVSNKA LPAPIEKTIS KAKGQPREPQ VYTLPPSRDE LTKNQVSLTC LVKGFYPSDI AVEWESNGQP ENNYKTTPPV LDSDGSFFLY SKLTVDKSRW QQGNVFSCSV MHEALHNHYT QKSLSLSPGK HHHHHH.
Product Science Overview
Structure and Expression
HVEM is broadly expressed on various immune cells, including T cells, natural killer (NK) cells, monocytes, and immature dendritic cells. It is also found in several non-hematopoietic tissues such as the liver, kidney, and lung . The recombinant form of HVEM, known as HVEM-Fc, is produced in Sf9 insect cells and consists of 146 amino acids (L39-K184) .
Functional Interactions
HVEM interacts with multiple ligands, including:
- LIGHT (TNFSF14): This interaction forms a hexameric complex that recruits and retains effector cells, leading to the activation of NK cells and the production of large amounts of IFN-γ and GM-CSF .
- BTLA (B- and T-lymphocyte attenuator): This interaction suppresses immune responses by inhibiting T- and B-lymphocyte activation and proliferation .
- CD160: This interaction also inhibits T- and B-lymphocyte activation and proliferation .
- HSV-gD (Herpes Simplex Virus glycoprotein D): This interaction promotes HSV infection in target cells .
Biological Activity
HVEM-Fc exhibits significant biological activity, including:
- Proinflammatory and Survival Signals: When engaged by BTLA or LIGHT, HVEM delivers proinflammatory and survival signals, stimulating lymphocyte proliferation and activation, and inducing inflammatory reactions .
- Inhibitory Signals: When bound to CD160 and BTLA, HVEM inhibits T- and B-lymphocyte activation and proliferation .
Applications
Production and Stability
HVEM-Fc is produced in Sf9 insect cells and is typically lyophilized after extensive dialysis against PBS. Upon reconstitution, it remains stable for up to 1 week at 4°C or up to 3 months at -20°C. For long-term storage, it is recommended to add a carrier protein, such as 0.1% BSA, to avoid repeated freeze-thaw cycles .
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