TNFRSF14 Human
Description
Product Specs
Product Science Overview
HVEM-Fc (Human Recombinant) is a fusion protein that combines the extracellular domain of the Herpesvirus Entry Mediator (HVEM) with the Fc region of human immunoglobulin G1 (IgG1). This recombinant protein is used in various research applications, particularly in immunology and virology, due to its role in modulating immune responses.
Herpesvirus Entry Mediator (HVEM), also known as Tumor Necrosis Factor Receptor Superfamily Member 14 (TNFRSF14) and CD270, is a type I transmembrane protein belonging to the TNF receptor superfamily . HVEM is expressed on various immune cells, including T cells, B cells, natural killer (NK) cells, monocytes, and neutrophils . It plays a crucial role in both promoting and inhibiting T cell activity .
The mature human HVEM protein consists of a 164 amino acid extracellular domain (ECD) with three cysteine-rich domains (CRDs), a 21 amino acid transmembrane segment, and a 60 amino acid cytoplasmic tail containing a TRAF interaction domain . The extracellular domain of HVEM is responsible for binding to its ligands, such as LIGHT (TNFSF14), BTLA (B and T Lymphocyte Attenuator), and CD160 .
The Fc region of human immunoglobulin G1 (IgG1) is a constant region that mediates various effector functions, including antibody-dependent cellular cytotoxicity (ADCC) and complement-dependent cytotoxicity (CDC). The Fc region also enhances the stability and half-life of the fusion protein in vivo .
The HVEM-Fc fusion protein is created by fusing the extracellular domain of HVEM to the Fc region of human IgG1. This fusion enhances the stability and solubility of HVEM, making it suitable for various experimental applications . The recombinant HVEM-Fc protein is typically produced in mammalian cell lines, such as HEK293 or NS0 cells, to ensure proper folding and post-translational modifications .
HVEM-Fc is widely used in research to study the interactions between HVEM and its ligands. It is particularly useful in investigating the modulation of immune responses, as HVEM can both activate and inhibit T cell activity depending on the context . Additionally, HVEM-Fc is employed in assays to block or neutralize the activity of HVEM ligands, providing insights into the mechanisms of immune regulation .
