TCEAL8 Human

Transcription Elongation Factor A (SII)-Like 8 Human Recombinant
Cat. No.
BT15309
Source
E.coli.
Synonyms
Transcription elongation factor A protein-like 8, TCEA-like protein 8, Transcription elongation factor S-II protein-like 8, TCEAL8.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 90% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

TCEAL8 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 125 amino acids (1-117 a.a) and having a molecular mass of 14.7kDa (Molecular weight on SDS-PAGE will appear higher).
TCEAL8 is fused to an 8 amino acid His-tag at C-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
Transcription elongation factor A protein-like 8 (TCEAL8), a member of the TFS-II family and TFA subfamily, plays a role in transcriptional regulation. This nuclear-localized protein belongs to the TFS-II family, whose members are characterized by their TFA domains and function as nuclear phosphoproteins. These proteins exert control over transcription in a promoter context-dependent manner. Notably, a significant number of family members are located on the X chromosome.
Description
Recombinant TCEAL8 Human, produced in E. coli, is a single, non-glycosylated polypeptide chain consisting of 125 amino acids (specifically, amino acids 1-117). It has a molecular mass of 14.7 kDa. However, the molecular weight observed on SDS-PAGE might appear higher. This TCEAL8 protein is fused to an 8 amino acid His-tag at the C-terminus and purified using proprietary chromatographic techniques.
Physical Appearance
A clear, sterile-filtered solution.
Formulation
The TCEAL8 protein solution (0.5 mg/ml) is supplied in a buffer containing 20 mM Tris-HCl (pH 8.0), 20% glycerol, 1 mM DTT, and 200 mM NaCl.
Stability
For short-term storage (up to 2-4 weeks), store the solution at 4°C. For extended storage, freeze the solution at -20°C. Adding a carrier protein (0.1% HSA or BSA) is recommended for long-term storage. It is important to avoid repeated freeze-thaw cycles.
Purity
The purity of the protein is determined to be greater than 90% by SDS-PAGE analysis.
Synonyms
Transcription elongation factor A protein-like 8, TCEA-like protein 8, Transcription elongation factor S-II protein-like 8, TCEAL8.
Source
E.coli.
Amino Acid Sequence
MQKSCEENEG KPQNMPKAEE DRPLEDVPQE AEGNPQPSEE GVSQEAEGNP RGGPNQPGQG FKEDTPVRHL DPEEMIRGVD ELERLREEIR RVRNKFVMMH WKQRHSRSRP YPVCFRPLEH HHHHH.

Product Science Overview

Introduction

Transcription Elongation Factor A (SII)-Like 8, also known as TCEA1, is a protein encoded by the TCEA1 gene in humans. This protein plays a crucial role in the process of transcription elongation, which is a vital step in gene expression. The recombinant form of this protein is often used in research to study its function and interactions.

Gene and Protein Structure

The TCEA1 gene is located on chromosome 8 at the band 8q11.23 . The gene encodes a protein that is part of the transcription factor TFIID complex, which is essential for the initiation and regulation of transcription by RNA polymerase II . The protein has several important domains that enable it to bind to DNA and interact with other proteins involved in transcription .

Function

The primary function of TCEA1 is to facilitate the elongation phase of transcription by RNA polymerase II. During transcription, RNA polymerase II can encounter obstacles that cause it to pause or backtrack. TCEA1 helps to resolve these issues by inducing a cut in the RNA polymerase active site, allowing transcription to resume from the new 3’-terminus . This activity is crucial for the efficient and accurate transcription of genes.

Biological Processes

TCEA1 is involved in several key biological processes, including:

  • Positive regulation of transcription by RNA polymerase II: TCEA1 enhances the transcriptional activity of RNA polymerase II, ensuring that genes are transcribed efficiently .
  • Transcription-coupled nucleotide excision repair (TC-NER): TCEA1 plays a role in the repair of DNA damage that occurs during transcription .
  • Erythrocyte differentiation: TCEA1 is involved in the differentiation of erythrocytes, which are red blood cells .
Interactions

TCEA1 interacts with several other proteins involved in transcription. Notably, it interacts with GTF2H1 and POLR2A, which are components of the transcription machinery . These interactions are essential for the proper functioning of the transcription elongation process.

Clinical Significance

Mutations or dysregulation of the TCEA1 gene can lead to various diseases. For example, TCEA1 has been associated with Calvarial Doughnut Lesions with Bone Fragility and Van Der Woude Syndrome . Understanding the function and regulation of TCEA1 is therefore important for developing potential therapeutic strategies for these conditions.

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