TCEAL1 Human

Transcription Elongation Factor A (SII)-Like 1 Human Recombinant
Cat. No.
BT15012
Source
Escherichia Coli.
Synonyms
Transcription elongation factor A protein-like 1, TCEA-like protein 1, Nuclear phosphoprotein p21/SIIR, Transcription elongation factor S-II protein-like 1, TCEAL1, SIIR, p21, pp21.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 90.0% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

TCEAL1 Human Recombinant fused with an 8 amino acid His tag at C-terminus produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 167 amino acids (1-159 a.a.) and having a molecular mass of 19.7kDa.
The TCEAL1 is purified by proprietary chromatographic techniques.

Product Specs

Introduction
Transcription elongation factor A protein-like 1 (TCEAL1), a member of the transcription elongation factor A (SII)-like (TCEAL) family, potentially acts as a nuclear phosphoprotein. It is believed to influence transcription in a way that depends on the promoter context. TCEAL1 plays a role in transcriptional regulation and exhibits expression in all tissues. Notably high expression levels are found in the heart, ovary, prostate, and skeletal muscle.
Description
Recombinant human TCEAL1, with an 8 amino acid His tag fused at its C-terminus, is produced in E. coli. It is a single, non-glycosylated polypeptide chain consisting of 167 amino acids (1-159 a.a.) and has a molecular weight of 19.7 kDa. The purification of TCEAL1 is achieved using proprietary chromatographic techniques.
Physical Appearance
A clear and colorless solution, sterile-filtered.
Formulation
The TCEAL1 solution has a concentration of 1 mg/ml and is prepared in a buffer containing 20mM Tris-HCl (pH 8.0), 2mM DTT, 20% glycerol, and 0.1M NaCl.
Stability
For short-term storage (2-4 weeks), the solution should be kept at 4°C. For extended storage, it is recommended to freeze the solution at -20°C. To ensure long-term stability during storage, adding a carrier protein (0.1% HSA or BSA) is advisable. It's important to minimize repeated freeze-thaw cycles.
Purity
The purity of the protein is greater than 90%, as determined by SDS-PAGE analysis.
Synonyms
Transcription elongation factor A protein-like 1, TCEA-like protein 1, Nuclear phosphoprotein p21/SIIR, Transcription elongation factor S-II protein-like 1, TCEAL1, SIIR, p21, pp21.
Source
Escherichia Coli.
Amino Acid Sequence
MDKPRKENEE EPQSAPKTDE ERPPVEHSPE KQSPEEQSSE EQSSEEEFFP EELLPELLPE MLLSEERPPQ EGLSRKDLFE GRPPMEQPPC GVGKHKLEEG SFKERLARSR PQFRGDIHGR NLSNEEMIQA ADELEEMKRV RNKLMIMHWK AKRSRPYPIL EHHHHHH.

Product Science Overview

Introduction

Transcription Elongation Factor A (SII)-Like 1, also known as TCEANC, is a protein-coding gene that plays a crucial role in the transcription process. This protein is involved in the elongation phase of transcription, which is a critical step in the synthesis of RNA from a DNA template. The human recombinant form of this protein is used in various research and therapeutic applications.

Gene and Protein Structure

The TCEANC gene encodes the Transcription Elongation Factor A (SII)-Like 1 protein. This gene is located on chromosome 1 and is composed of multiple exons that are transcribed into mRNA. The protein itself consists of several domains that are essential for its function in transcription elongation. These domains include the N-terminal and central domains, which are involved in the interaction with RNA polymerase II and other transcription factors .

Function

The primary function of Transcription Elongation Factor A (SII)-Like 1 is to facilitate the elongation phase of transcription by RNA polymerase II. During transcription, RNA polymerase II can encounter obstacles that cause it to pause or backtrack. TCEANC helps to resolve these issues by inducing cleavage of the nascent RNA, allowing transcription to resume from the new 3’-terminus . This activity is crucial for the efficient and accurate synthesis of RNA molecules.

Biological Significance

Transcription Elongation Factor A (SII)-Like 1 is essential for the proper regulation of gene expression. By ensuring the smooth progression of RNA polymerase II during transcription, TCEANC plays a vital role in the expression of genes involved in various cellular processes. Dysregulation of this protein can lead to transcriptional errors and has been associated with certain diseases, including infantile myofibromatosis .

Applications

The human recombinant form of Transcription Elongation Factor A (SII)-Like 1 is used in research to study the mechanisms of transcription elongation and its regulation. It is also utilized in therapeutic applications to investigate potential treatments for diseases caused by transcriptional dysregulation. The availability of recombinant TCEANC allows researchers to conduct experiments with a consistent and reliable source of the protein.

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Source
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