sRANKL (158-316) Mouse
Greater than 90% as determined by SDS-PAGE.
Description
sRANKL Mouse Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 160 amino acids (158-316 a.a.) and having a molecular mass of 17.9kDa. sRANKL is purified by proprietary chromatographic techniques.
Product Specs
Recombinant Mouse sRANKL, produced in E. coli, is a single, non-glycosylated polypeptide chain encompassing amino acids 158-316. With a molecular weight of 17.9 kDa, it is purified using proprietary chromatographic techniques.
This product consists of sRANKL protein solution at a concentration of 1 mg/ml. It is formulated in Tris-HCl buffer with a pH of 8.5 and 0.1M NaCl.
For short-term storage (2-4 weeks), the product should be kept at 4°C. For extended periods, storage at -20°C is recommended. Adding a carrier protein (0.1% HSA or BSA) is advisable for long-term storage. Repeated freezing and thawing should be avoided.
The purity of this product is greater than 90% as determined by SDS-PAGE analysis.
MKPEAQPFAH LTINAASIPS GSHKVTLSSW YHDRGWAKIS NMTLSNGKLR VNQDGFYYLY ANICFRHHET SGSVPTDYLQ LMVYVVKTSI KIPSSHNLMK GGSTKNWSGN SEFHFYSINV GGFFKLRAGE EISIQVSNPS LLDPDQDATY FGAFKVQDID
Product Science Overview
Soluble Receptor Activator of Nuclear Factor Kappa-B Ligand (sRANKL) is a crucial protein involved in bone metabolism and immune system regulation. The specific variant, Soluble RANK Ligand (158-316 a.a) (Mouse Recombinant), is a recombinant protein produced in Escherichia coli (E. coli) and is widely used in research to study its biological functions and therapeutic potential.
The Soluble RANK Ligand (158-316 a.a) (Mouse Recombinant) is a non-glycosylated polypeptide chain consisting of 159 amino acids, with a molecular mass of approximately 19 kDa . The protein is expressed in E. coli and purified using proprietary chromatographic techniques to achieve a purity greater than 95% as determined by SDS-PAGE .
sRANKL binds to its receptors, TNFRSF11B (osteoprotegerin) and TNFRSF11A (RANK), playing a pivotal role in osteoclast differentiation and activation . This interaction is essential for bone resorption and remodeling. Additionally, sRANKL enhances the ability of dendritic cells to stimulate naive T-cell proliferation, indicating its importance in the regulation of T-cell-dependent immune responses .
The recombinant sRANKL is used extensively in research to study its effects on bone metabolism and immune system regulation. It is particularly valuable in investigating conditions such as osteoporosis, rheumatoid arthritis, and cancer-induced bone diseases. The protein’s ability to induce osteoclast differentiation makes it a critical tool for understanding bone resorption mechanisms and developing potential therapeutic interventions .
Lyophilized sRANKL is stable at room temperature for up to three weeks but should be stored desiccated below -18°C for long-term stability . Upon reconstitution, the protein should be stored at 4°C for short-term use (2-7 days) and below -18°C for long-term use. To prevent freeze-thaw cycles, it is recommended to add a carrier protein such as human serum albumin (HSA) or bovine serum albumin (BSA) .
