sRANKL Human, His
Soluble Receptor Activator of NFkB Ligand, TNFSF11, TRANCE, TNF-related activation-induced cytokine, OPGL, ODF, Osteoclast differentiation factor, Tumor necrosis factor ligand superfamily member 11, Receptor activator of nuclear factor kappa B ligand, RANKL, Osteoprotegerin ligand, CD254 antigen, sRANKL, sOdf, hRANKL2.
Description
sRANKL Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 199 amino acids (140-317 a.a) and having a molecular mass of 22.3kDa.
sRANKL is fused to a 21 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Product Specs
Soluble Receptor Activator of NFkB Ligand, TNFSF11, TRANCE, TNF-related activation-induced cytokine, OPGL, ODF, Osteoclast differentiation factor, Tumor necrosis factor ligand superfamily member 11, Receptor activator of nuclear factor kappa B ligand, RANKL, Osteoprotegerin ligand, CD254 antigen, sRANKL, sOdf, hRANKL2.
MGSSHHHHHH SSGLVPRGSH MIRAEKAMVD GSWLDLAKRS KLEAQPFAHL TINATDIPSG SHKVSLSSWY HDRGWAKISN MTFSNGKLIV NQDGFYYLYA NICFRHHETS GDLATEYLQL MVYVTKTSIK IPSSHTLMKG GSTKYWSGNS EFHFYSINVG GFFKLRSGEE ISIEVSNPSL LDPDQDATYF GAFKVRDID.
Product Science Overview
The recombinant human sRANKL is typically produced in Escherichia coli (E. coli) and purified through sequential chromatography . The protein is a non-glycosylated polypeptide chain containing 199 amino acids, with a molecular mass of approximately 22.3 kDa . The His tag, consisting of 21 amino acids, is fused to the N-terminus of the protein to facilitate purification and detection .
sRANKL is known for its ability to bind to the receptor activator of nuclear factor kappa-B (RANK) on the surface of osteoclast precursors, promoting their differentiation into mature osteoclasts. This interaction is essential for bone resorption and remodeling . Additionally, sRANKL can interact with a decoy receptor called osteoprotegerin (OPG), which inhibits osteoclastogenesis by preventing sRANKL from binding to RANK .
Recombinant sRANKL is used extensively in cell culture, differentiation studies, and functional assays. It is particularly valuable in research focused on bone diseases, such as osteoporosis and rheumatoid arthritis, as well as in studies of immune system regulation . The protein’s ability to induce NF-κB activation in various cell types makes it a powerful tool for investigating signaling pathways involved in inflammation and immune responses .
The lyophilized form of sRANKL is typically reconstituted in distilled water to a concentration of 0.1-1.0 mg/mL . For long-term storage, it is recommended to store the reconstituted solution in working aliquots at -80°C to -20°C, with a carrier protein such as 0.1% bovine serum albumin (BSA) to enhance stability . The lyophilized protein is stable at room temperature for up to one month but should be stored at -20°C for extended periods .
