sRANKL Human, GST

The human recombinant sRANKL fused with a Glutathione S-Transferase (GST) tag is produced in E. coli. This recombinant protein is a single, non-glycosylated polypeptide with a molecular mass of approximately 47 kDa . The GST tag aids in the purification process, making it easier to isolate the protein using affinity chromatography techniques .

RANKL is essential for the formation, function, and survival of osteoclasts, which are cells responsible for bone resorption. It binds to its receptor, RANK, on the surface of osteoclast precursors, promoting their differentiation into mature osteoclasts . This interaction is critical for maintaining bone homeostasis and remodeling.

In addition to its role in bone metabolism, RANKL is involved in the immune system. It is expressed by T cells and plays a role in the development of lymph nodes and the regulation of immune responses .

The recombinant form of sRANKL with a GST tag is widely used in research to study its biological functions and interactions. It is particularly valuable in:

• Bone Metabolism Studies: Understanding the mechanisms of bone diseases such as osteoporosis and rheumatoid arthritis.
• Cancer Research: Investigating the role of RANKL in cancer metastasis, especially in bone metastasis.
• Immunology: Exploring the functions of RANKL in immune cell regulation and lymph node development .

The soluble RANKL protein solution is typically formulated with 1mM EDTA and PBS at pH 7.4. It should be stored at -20°C for long-term storage and at 4°C if used within 2-4 weeks. It is important to avoid freeze-thaw cycles to maintain the protein’s stability .