RANK Human, Sf9

The human recombinant RANK protein is produced using the Sf9 insect cell line derived from the fall armyworm, Spodoptera frugiperda. This cell line is commonly used in biotechnology for the expression of recombinant proteins. The production process involves the use of a baculovirus expression system, which is a popular method for producing high-quality recombinant proteins.

RANK, along with its ligand RANKL (Receptor Activator of Nuclear Factor Kappa-Β Ligand), is essential for the formation, function, and survival of osteoclasts, the cells responsible for bone resorption. The interaction between RANK and RANKL activates signaling pathways that lead to the differentiation and activation of osteoclasts, which in turn regulate bone remodeling and calcium homeostasis.

RANK is also expressed on dendritic cells, which are key players in the immune system. The RANK-RANKL interaction is involved in the maturation and activation of dendritic cells, influencing the immune response. This interaction is crucial for the development of lymph nodes and the formation of secondary lymphoid organs.

In the mammary gland, RANK signaling is vital for the proliferation and differentiation of mammary epithelial cells during pregnancy. This process is essential for the development of the mammary gland and the production of milk.

Given its significant roles in bone metabolism and the immune system, RANK is a target for therapeutic interventions in various diseases. For instance, inhibitors of the RANK-RANKL interaction, such as denosumab, are used in the treatment of osteoporosis and bone metastases in cancer patients. Additionally, modulating RANK signaling pathways holds potential for treating autoimmune diseases and enhancing immune responses.