SNRPD3 Human, Sf9
Description
SNRPD3 is expressed with a -6x His tag at N-terminus and purified by proprietary chromatographic techniques.
Product Specs
Product Science Overview
SNRPD3 is a small nuclear ribonucleoprotein that forms part of the core of the spliceosomal snRNPs, including U1, U2, U4, and U5 . These snRNPs are the building blocks of the spliceosome, which is involved in the removal of introns from pre-mRNA transcripts . The protein is also a component of the minor spliceosome, which is responsible for the splicing of U12-type introns .
The human recombinant version of SNRPD3 produced in Sf9 insect cells is a glycosylated polypeptide chain with a calculated molecular mass of approximately 14,739 Daltons . It is expressed with a 6x His tag at the N-terminus and purified using proprietary chromatographic techniques .
The recombinant SNRPD3 protein is used in various laboratory research applications, including:
- Western Blotting: It is used with anti-Sm positive patient sera to detect the presence of auto-antibodies .
- ELISA: The protein is suitable for standard ELISA tests, including checker-board analysis of positive/negative sera panels .
- Immunological Studies: It binds IgG-type human auto-antibodies, making it useful for immunological research .
The recombinant SNRPD3 protein is supplied in a sterile filtered clear solution and is formulated in 20mM HEPES buffer (pH 7.6), 250mM NaCl, and 20% glycerol . For optimal stability, it should be stored at 4°C if used within 2-4 weeks or frozen at -20°C for longer periods. It is important to avoid multiple freeze-thaw cycles to maintain its integrity .
