SNRPD1 Human

SNRPD1 belongs to the SNRNP core protein family. It plays a significant role in the assembly and stability of snRNPs by acting as a charged protein scaffold. This scaffold promotes snRNP assembly and strengthens snRNP-snRNP interactions through nonspecific electrostatic contacts with RNA . The protein is involved in the formation of the spliceosome, a complex responsible for the removal of introns from pre-mRNA .

The SNRPD1 gene is located on chromosome 18 in humans . It has been found to have two transcript variants encoding different isoforms of the protein . The gene is expressed in various tissues, including the ganglionic eminence, oocyte, oral cavity, islet of Langerhans, appendix, trabecular bone, bone marrow, superior surface of the tongue, human penis, and pylorus .

SNRPD1 interacts with several other proteins, including CDC5L, CLNS1A, DDX20, SMN1, and Small Nuclear Ribonucleoprotein D2 . These interactions are crucial for the proper functioning of the spliceosome and the splicing process.

Human recombinant SNRPD1 is produced in SF9 insect cells. The recombinant protein is glycosylated and has a calculated molecular mass of 16 kDa . It is expressed with a 6x His tag at the N-terminus and purified using proprietary chromatographic techniques .

Mutations or dysregulation of the SNRPD1 gene have been associated with various diseases, including systemic lupus erythematosus and autoimmune diseases . The protein’s role in RNA splicing makes it a critical factor in maintaining cellular function and gene expression.