SNRPD Human
Description
Each protein (SNRPD1+ SNRPD2+ SNRPD3) is individually fused to a -6xHis tag at N-terminus and purified by proprietary chromatographic techniques.
Product Specs
Product Science Overview
Small nuclear ribonucleoproteins (snRNPs) are essential components of the spliceosome, the complex responsible for pre-mRNA splicing in eukaryotic cells. Among these, the D1, D2, and D3 polypeptides play crucial roles in the assembly and function of snRNPs. This article delves into the background, structure, and significance of these polypeptides, particularly focusing on their human recombinant forms.
The small nuclear ribonucleoprotein polypeptides D1, D2, and D3 are part of the core protein family of snRNPs. These proteins are integral to the formation of the spliceosomal U1, U2, U4, and U5 snRNPs, which are the building blocks of the spliceosome .
- D1 Polypeptide: Encoded by the SNRPD1 gene, the D1 polypeptide acts as a charged protein scaffold, promoting snRNP assembly and strengthening snRNP-snRNP interactions through nonspecific electrostatic contacts with RNA . It is involved in the splicing of U12-type introns in pre-mRNAs .
- D2 Polypeptide: Encoded by the SNRPD2 gene, the D2 polypeptide is required for pre-mRNA splicing and snRNP biogenesis . It interacts with other snRNP components to facilitate the assembly and function of the spliceosome.
- D3 Polypeptide: Encoded by the SNRPD3 gene, the D3 polypeptide shares structural similarities with D1 and D2 and is involved in the same processes . It plays a role in the formation of the snRNP core and the overall stability of the spliceosome.
Human recombinant forms of these polypeptides are produced using recombinant DNA technology, which involves inserting the gene encoding the polypeptide into a suitable expression system, such as bacteria or yeast. This allows for the production of large quantities of the protein, which can be purified and used for various research and therapeutic purposes.
Mutations or dysregulation of the genes encoding these polypeptides have been associated with various diseases, including systemic lupus erythematosus and other autoimmune disorders . Understanding the structure and function of these polypeptides is crucial for developing targeted therapies for these conditions.
