SNRPD2 Human, Sf9

Small Nuclear Ribonucleoprotein Polypeptide D2 (SNRPD2) is a protein encoded by the SNRPD2 gene in humans. This protein is a crucial component of the small nuclear ribonucleoprotein (snRNP) core protein family, which plays a significant role in the splicing of pre-mRNA. The splicing process is essential for the maturation of messenger RNA (mRNA), which is necessary for the translation of genetic information into functional proteins .

SNRPD2 is involved in the assembly and function of the spliceosome, a complex molecular machine responsible for removing introns from pre-mRNA. The spliceosome is composed of several snRNPs, including U1, U2, U4, U5, and U6, each containing specific snRNP proteins like SNRPD2. This protein is required for the biogenesis of snRNPs and the proper splicing of pre-mRNA .

The human recombinant SNRPD2 protein can be produced using various expression systems, including the Sf9 insect cell system. The Sf9 cells, derived from the fall armyworm (Spodoptera frugiperda), are commonly used for the production of recombinant proteins due to their high expression levels and ability to perform post-translational modifications. The recombinant SNRPD2 protein is typically fused with a tag, such as a His-tag, to facilitate purification using chromatographic techniques .

SNRPD2 interacts with several other proteins and components within the spliceosome. It has been shown to interact with DDX20, Small Nuclear Ribonucleoprotein D1, Small Nuclear Ribonucleoprotein Polypeptide F, CDC5L, and SMN1 . These interactions are crucial for the assembly and function of the spliceosome, ensuring accurate and efficient splicing of pre-mRNA.

The analysis of SNRPD2 and its interactions can be performed using various biochemical and biophysical techniques. These include co-immunoprecipitation, mass spectrometry, and X-ray crystallography. These methods help elucidate the structure and function of SNRPD2 within the spliceosome and its role in pre-mRNA splicing .