SNRPD3 Human
Small Nuclear Ribonucleoprotein Polypeptide D3 Human Recombinant
Cat. No.
BT1343
Source
E.coli.
Synonyms
Small nuclear ribonucleoprotein D3 polypeptide 18kDa, Sm-D3, snRNP core protein D3.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 85% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description
SNRPD3 Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 146 amino acids (1-126) and having a molecular mass of 16.0 kDa.
The SNRPD3 is fused to a 20 amino acid His-Tag at N-terminus and purified by proprietary chromatographic techniques.
The SNRPD3 is fused to a 20 amino acid His-Tag at N-terminus and purified by proprietary chromatographic techniques.
Product Specs
Introduction
SNRPD3, a small nuclear ribonucleoprotein (snRNP), is a component of the spliceosome in eukaryotic cells. This protein plays a crucial role in pre-mRNA splicing and the biogenesis of small nuclear ribonucleoproteins. Two transcript variants encoding the same SNRPD3 protein have been identified due to alternative splicing.
Description
Recombinant SNRPD3, produced in E. coli, is a single, non-glycosylated polypeptide chain consisting of 146 amino acids (residues 1-126). It has a molecular weight of 16.0 kDa. For purification purposes, a 20 amino acid His-Tag is fused to the N-terminus. The protein is purified using proprietary chromatographic techniques.
Physical Appearance
Clear, colorless solution, sterile filtered.
Formulation
The SNRPD3 solution is provided at a concentration of 0.25 mg/ml in a buffer containing 20 mM Tris-HCl (pH 8.0), 0.5 M NaCl, 2 mM DTT, 0.1 mM PMSF, and 40% glycerol.
Stability
For short-term storage (2-4 weeks), the product can be stored at 4°C. For extended storage, it is recommended to freeze the product at -20°C. To ensure long-term stability during frozen storage, the addition of a carrier protein (0.1% HSA or BSA) is advisable. Avoid repeated freeze-thaw cycles.
Purity
The purity of SNRPD3 is greater than 85%, as determined by SDS-PAGE analysis.
Synonyms
Small nuclear ribonucleoprotein D3 polypeptide 18kDa, Sm-D3, snRNP core protein D3.
Source
E.coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MSIGVPIKVL HEAEGHIVTC ETNTGEVYRG KLIEAEDNMN CQMSNITVTY RDGRVAQLEQ VYIRGSKIRF LILPDMLKNA PMLKSMKNKN QGSGAGRGKA AILKAQVAAR GRGRGMGRGN IFQKRR
Product Science Overview
Function and Importance
SNRPD3 belongs to the small nuclear ribonucleoprotein (snRNP) core protein family . It plays a vital role in the splicing of pre-mRNA, which is a critical step in the post-transcriptional modification of RNA . The spliceosome, composed of snRNPs including SNRPD3, removes introns from pre-mRNA and joins exons together to form mature mRNA .
Gene and Protein Structure
Biological Pathways
SNRPD3 is involved in several biological pathways, including:
- Processing of Capped Intron-Containing Pre-mRNA
- Processing of Capped Intronless Pre-mRNA
- Histone pre-mRNA 3’-end processing
These pathways are essential for the proper expression and regulation of genes, impacting various cellular processes and functions .
Clinical Significance
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