SNAPIN Human

SNAP Associated Protein Human Recombinant
Cat. No.
BT6502
Source
Escherichia Coli.
Synonyms
SNAPAP, SNARE-associated protein Snapin, Synaptosomal-associated protein 25-binding protein, SNAP-associated protein, SNAPIN, SNAP25BP.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 90.0% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

SNAPIN Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 156 amino acids (1-136) and having a molecular mass of 17 kDa. SNAPIN is fused to 20 amino acid His Tag at N-terminus and purified by standard chromatography techniques.

Product Specs

Introduction
SNAPIN plays a crucial role in the release of neurotransmitters by regulating the interactions between SNARE proteins and synaptotagmin, which are essential for synaptic vesicle fusion and neurotransmitter release. As a component of the SNARE complex, SNAPIN is vital for synaptic vesicle docking and fusion. Primarily found in neurons, SNAPAP is exclusively located on synaptic vesicle membrane protein. Moreover, SNAPIN is a key component of the BLOC1 protein complex, which is essential for the proper formation of specialized organelles within the endosomal-lysosomal system, including melanosomes and platelet dense granules.
Description
Recombinant Human SNAPIN, produced in E. coli, is a single, non-glycosylated polypeptide chain consisting of 156 amino acids (residues 1-136) with a molecular weight of 17 kDa. The protein is expressed with a 20 amino acid His Tag at the N-terminus and purified using standard chromatography techniques.
Physical Appearance
The product is a sterile-filtered solution, colorless and transparent.
Formulation
The SNAPIN protein is supplied in a solution containing 20mM Tris-HCl (pH 8.0), 5mM DTT, 2mM EDTA, 0.2M NaCl, and 40% glycerol.
Stability
For short-term storage (2-4 weeks), the product should be stored at 4°C. For long-term storage, it is recommended to store the protein at -20°C. To ensure stability during long-term storage, adding a carrier protein (0.1% HSA or BSA) is advised. Avoid repeated freeze-thaw cycles to prevent protein degradation.
Purity
The purity of SNAPIN is greater than 90% as determined by SDS-PAGE analysis.
Synonyms
SNAPAP, SNARE-associated protein Snapin, Synaptosomal-associated protein 25-binding protein, SNAP-associated protein, SNAPIN, SNAP25BP.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MAGAGSAAVS GAGTPVAGPT GRDLFAEGLL EFLRPAVQQL DSHVHAVRES QVELREQIDN LATELCRINEDQKVALDLDP YVKKLLNARR RVVLVNNILQ NAQERLRRLN HSVAKETARR RAMLDSGIYP PGSPGK.

Product Science Overview

Introduction

Synaptosomal-Associated Protein 25 (SNAP-25) is a crucial protein involved in the regulation of neurotransmitter release at synapses. It is encoded by the SNAP25 gene located on chromosome 20p12.2 in humans . This protein is a member of the SNAP-25 family and plays a significant role in the SNARE (Soluble NSF Attachment Protein Receptor) complex, which is essential for synaptic vesicle fusion and neurotransmitter release .

Structure and Function

SNAP-25 is a membrane-bound protein anchored to the cytosolic face of membranes via palmitoyl side chains in the middle of the molecule . It contains two t-SNARE coiled-coil homology domains, which are critical for its function in the SNARE complex . The SNARE complex is responsible for the specificity of membrane fusion and directly executes fusion by forming a tight complex that brings the synaptic vesicle and plasma membranes together .

Recombinant SNAP-25

Recombinant human SNAP-25 is produced using Escherichia coli (E. coli) as the host organism . The recombinant protein consists of 217 amino acids and has a calculated molecular mass of 24.8 kDa, although it migrates as an approximately 28 kDa band in SDS-PAGE under reducing conditions . The protein is typically lyophilized from sterile PBS, pH 7.4, and may contain protectants such as trehalose, mannitol, and Tween80 . It is stable for up to twelve months when stored at -20°C to -80°C under sterile conditions .

Biological Significance

SNAP-25 is a key component of the SNARE complex, which includes syntaxin-1 and synaptobrevin . This complex is essential for the exocytotic fusion of synaptic vesicles with the presynaptic membrane, a process critical for neurotransmitter release . SNAP-25 has been implicated in various neurological disorders, including Attention Deficit Hyperactivity Disorder (ADHD), schizophrenia, bipolar disorder, and epilepsy . Its role in these conditions highlights its importance as a shared biological substrate among different "synaptopathies" .

Clinical Relevance

Mutations or dysregulation of the SNAP25 gene can lead to developmental and epileptic encephalopathies (DEEs), learning disabilities, and other neurological conditions . The protein’s involvement in synaptic vesicle docking and neurotransmitter release makes it a potential target for therapeutic interventions in these disorders .

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