SNAP25 Human
Description
Product Specs
Adding a carrier protein (0.1% HSA or BSA) is recommended for long-term storage.
Avoid repeated freezing and thawing.
Product Science Overview
Synaptosomal-Associated Protein 25kDa (SNAP-25) is a crucial protein involved in the regulation of neurotransmitter release at synapses. It is a member of the SNARE (Soluble NSF Attachment Protein Receptor) complex, which plays a pivotal role in the fusion of synaptic vesicles with the presynaptic membrane, facilitating the release of neurotransmitters into the synaptic cleft .
SNAP-25 is encoded by the SNAP25 gene located on chromosome 20p12.2 in humans . The protein consists of 206 amino acids and has a molecular mass of approximately 23 kDa . It is a non-glycosylated polypeptide chain produced in E. coli for recombinant purposes .
The primary function of SNAP-25 is to mediate synaptic vesicle exocytosis. It achieves this by forming a tight complex with other SNARE proteins, such as syntaxin-1 and synaptobrevin, which brings the synaptic vesicle and plasma membranes together . This complex formation is essential for the Ca²⁺-triggered fusion of synaptic vesicles, allowing neurotransmitters to be released into the synaptic cleft .
SNAP-25 exists in two isoforms, SNAP-25a and SNAP-25b, which result from alternative splicing of the exon 5 of the SNAP25 gene . SNAP-25a is predominantly expressed during the embryonic stage, while SNAP-25b becomes the major isoform during postnatal life . This developmental regulation ensures that SNAP-25 is appropriately expressed to meet the changing demands of synaptic transmission throughout development.
Alterations in SNAP-25 expression and function have been associated with various neurological and psychiatric disorders. For instance, mutations in the SNAP25 gene have been linked to developmental and epileptic encephalopathies (DEEs), Attention Deficit Hyperactivity Disorder (ADHD), schizophrenia, bipolar disorder, and Alzheimer’s disease . These associations highlight the critical role of SNAP-25 in maintaining normal synaptic function and its potential involvement in the pathophysiology of these conditions.
Human recombinant SNAP-25 is produced using E. coli expression systems. This recombinant protein is utilized in various research applications to study its structure, function, and role in synaptic transmission. The availability of recombinant SNAP-25 allows researchers to investigate its interactions with other proteins and its involvement in synaptic vesicle exocytosis in a controlled environment .
