SNAP25 C.elegans

Synaptosomal-associated protein 25kDa C.elegans Recombinant
Cat. No.
BT6328
Source
Escherichia Coli.
Synonyms
Super-Protein, SUP, RIC4, SEC9, SNAP, RIC-4, SNAP25, SNAP-25, Synaptosomal-associated protein 25, Synaptosomal-associated 25 kDa protein, FLJ23079, bA416N4.2, dJ1068F16.2.
Appearance
Sterile filtered colorless solution.
Purity
Greater than 95.0% as determined by SDS-PAGE.
Usage
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Shipped with Ice Packs
In Stock
Quick Inquiry

Description

Recombinant C.elegans SNAP-25 produced in E.Coli is a single, non-glycosylated polypeptide chain containing 207 amino acids and having a molecular mass of 23kDa.
SNAP-25 gene was amplified by PCR from C.elegans and cloned into an E. coli expression vector. SNAP-25 was purified by using conventional chromatography techniques.

Product Specs

Introduction
SNARE proteins (soluble N-ethylmaleimide-sensitive factor attachment protein receptors) are crucial for synaptic vesicle membrane docking and fusion. These proteins are located on both the vesicle membrane (v-SNAREs) and the target membrane (t-SNAREs). The interaction between v-SNAREs and t-SNAREs forms a complex composed of four helices. One helix originates from the v-SNARE, while the remaining three come from the t-SNARE. In the context of t-SNAREs found on the plasma membrane, the protein syntaxin provides one helix, and the protein encoded by the SNAP25 gene contributes the other two helices. This highlights the role of the SNAP25 protein, located on the presynaptic plasma membrane, in regulating neurotransmitter release. As a core component of the complex responsible for presynaptic vesicle trafficking, SNAP-25 (synaptosomal-associated protein) is directly involved in the release of neurotransmitters.
Description
This recombinant C. elegans SNAP-25 protein, expressed in E. coli, is a single, non-glycosylated polypeptide chain. It comprises 207 amino acids, resulting in a molecular weight of 23kDa. The SNAP-25 gene was amplified from C. elegans using PCR and subsequently cloned into an E. coli expression vector. Purification of the SNAP-25 protein was achieved through standard chromatography methods.
Physical Appearance
A clear, colorless solution that has been sterilized by filtration.
Formulation
This protein solution is formulated in a buffer containing 20mM Tris-HCl (pH 7.5), 50mM NaCl, 5mM DTT, 1mM EDTA, and 10% Glycerol.
Stability
For optimal storage, refrigerate the product at 4°C if it will be used within 2-4 weeks. For long-term storage, freeze the product at -20°C. Adding a carrier protein (0.1% HSA or BSA) is recommended for extended storage. Minimize repeated freeze-thaw cycles to maintain protein integrity.
Purity
The purity of this product exceeds 95.0%, as determined by SDS-PAGE analysis.
Synonyms
Super-Protein, SUP, RIC4, SEC9, SNAP, RIC-4, SNAP25, SNAP-25, Synaptosomal-associated protein 25, Synaptosomal-associated 25 kDa protein, FLJ23079, bA416N4.2, dJ1068F16.2.
Source
Escherichia Coli.
Amino Acid Sequence

MSGDDDIPEG LEAINLKMNA TTDDSLESTR RMLALCEESK EAGIKTLVML DDQGEQLERCEGALDTINQD MKEAEDHLKG MEKCCGLCVL PWNKTDDFEK TEFAKAWKKD DDGGVISDQPRITVGDSSMG PQGGYITKIT NDAREDEMDE NVQQVSTMVG NLRNMAIDMS TEVSNQNRQL DRIHDKAQSN EVRVESANKR AKNLITK.

Product Science Overview

Introduction

Synaptosomal-associated protein 25kDa (SNAP-25) is a crucial component of the SNARE (Soluble NSF Attachment Protein Receptor) complex, which plays a vital role in the exocytotic release of neurotransmitters during synaptic transmission. This protein is highly conserved across species, including the model organism Caenorhabditis elegans (C. elegans). The recombinant form of SNAP-25 from C. elegans has been extensively studied to understand its structure, function, and potential applications in neuroscience research.

Structure and Function

SNAP-25 is a presynaptic protein that is essential for the proper functioning of synaptic vesicle exocytosis. It forms a coiled-coil assembly with syntaxin-1 and synaptobrevin, facilitating the apposition of synaptic vesicles to the presynaptic membrane and permitting their calcium-triggered fusion . This process is critical for the release of neurotransmitters into the synaptic cleft, enabling neuronal communication.

The protein exists in two isoforms, SNAP-25a and SNAP-25b, which are differentially expressed during development. SNAP-25a is predominantly expressed during the embryonic stage, while SNAP-25b becomes the major isoform during postnatal life .

Recombinant SNAP-25 from C. elegans

Recombinant SNAP-25 from C. elegans is produced in Escherichia coli (E. coli) as a single, non-glycosylated polypeptide chain containing 207 amino acids and having a molecular mass of approximately 23kDa . The gene encoding SNAP-25 is amplified by PCR from C. elegans and cloned into an E. coli expression vector. The protein is then purified using conventional chromatography techniques .

Applications in Research

The recombinant form of SNAP-25 from C. elegans is widely used in laboratory procedures to study the protein’s role in synaptic transmission and its interactions with other cellular components. It is particularly valuable in research focused on understanding the molecular mechanisms underlying synaptic vesicle exocytosis and the regulation of neurotransmitter release.

Clinical Relevance

Alterations in SNAP-25 have been associated with various neurological and psychiatric disorders, including Attention Deficit Hyperactivity Disorder (ADHD), schizophrenia, and bipolar disorder . Understanding the function and regulation of SNAP-25 can provide insights into the pathophysiology of these conditions and potentially lead to the development of targeted therapies.

Quick Inquiry

Personal Email Detected
Please use an institutional or corporate email address for inquiries. Personal email accounts ( such as Gmail, Yahoo, and Outlook) are not accepted. *

Category

Service

Related Products

SNAP23 Human
Synaptosomal-associated protein 23 Human Recombinant
Cat. No.
BT6252
Source
Escherichia Coli.
SNAP25 Human
Synaptosomal-associated protein 25kda Human Recombinant
Cat. No.
BT6391
Source
Escherichia Coli.
SNAP25 Human, His
Synaptosomal-associated protein 25kDa Human Recombinant, His Tag
Cat. No.
BT6431
Source
Escherichia Coli.
SNAPIN Human
SNAP Associated Protein Human Recombinant
Cat. No.
BT6502
Source
Escherichia Coli.
THE BIOTEK
THE BioTek is a global biotech company offering highly purified proteins for research in cancer, apoptosis, development, endocrinology, immunology, neuroscience, proteases, and stem cells.
  • Contact
  • Address: 1925 E Maple Ave, El Segundo, CA 90245 United States
  • Phone : +1 201-285-7826
  • Email : info@thebiotek.com
  • Hours : Mon.-Fri. 9:00 AM - 5:00 PM
© Copyright 2024 Thebiotek. All Rights Reserved.