SNAP25 Human, His
Description
Product Specs
Product Science Overview
Synaptosomal-Associated Protein 25kDa (SNAP-25) is a crucial protein involved in the regulation of neurotransmitter release at synapses. It is encoded by the SNAP25 gene located on chromosome 20p12.2 in humans . The recombinant form of this protein, tagged with a His (histidine) tag, is often used in research to facilitate purification and detection.
SNAP-25 is a member of the SNARE (Soluble NSF Attachment Protein Receptor) protein family. It plays a pivotal role in the formation of the SNARE complex, which is essential for the fusion of synaptic vesicles with the plasma membrane . This fusion process is critical for the release of neurotransmitters into the synaptic cleft, thereby enabling neuronal communication.
The protein consists of 206 amino acids and has a molecular mass of approximately 25 kDa . The His tag, typically added to the N-terminus of the recombinant protein, consists of a series of histidine residues that bind to nickel ions, allowing for easy purification using nickel-affinity chromatography .
SNAP-25 is predominantly expressed in the brain, particularly in regions such as the cerebellum, cortex, and hippocampus . It is involved in various biological processes, including synaptic vesicle docking, neurotransmitter release, and synaptic plasticity . These functions are vital for learning, memory, and overall cognitive function.
Mutations or dysregulation of the SNAP25 gene have been associated with several neurological disorders. For instance, alterations in SNAP-25 expression or function have been linked to conditions such as schizophrenia, epilepsy, and attention deficit hyperactivity disorder (ADHD) . Additionally, SNAP-25 is a target for botulinum neurotoxins, which cleave the protein and inhibit neurotransmitter release, leading to muscle paralysis .
The recombinant form of SNAP-25, tagged with a His tag, is widely used in research to study its structure, function, and interactions with other proteins. The His tag facilitates the purification and detection of the protein, making it easier to conduct biochemical and biophysical analyses . This recombinant protein is also used in drug discovery and development, particularly in screening for compounds that modulate SNARE complex formation and function.
