SDF 1a Mouse
Greater than 95% as determined by SDS-PAGE.
Description
Stromal Cell-Derived Factor-1 alpha Mouse Recombinant produced in E.Coli is a non-glycosylated, Polypeptide chain containing 68 amino acids and having a molecular mass of 8 kDa.
The SDF-1a is purified by proprietary chromatographic techniques.
Product Specs
SDF-1, also known as stromal cell-derived factor-1, is a chemokine that plays a crucial role in directing cell migration and coordinating various cellular processes. It exists in two forms, SDF-1α (CXCL12a) and SDF-1β (CXCL12b), generated by alternative splicing of the CXCL12 gene. SDF-1 belongs to the CXC chemokine family, characterized by a specific arrangement of cysteine residues. It exhibits potent chemotactic activity, attracting lymphocytes and guiding the movement of hematopoietic cells during development. Notably, SDF-1 is essential for the homing of hematopoietic cells to the bone marrow during embryogenesis. Mice lacking the CXCL12 gene exhibit lethality either before or shortly after birth. Furthermore, SDF-1α influences neuronal electrophysiology. Its expression is observed in various tissues, including the brain, thymus, heart, lung, liver, kidney, spleen, and bone marrow. The primary receptor for SDF-1 is CXCR4, previously termed fusin. While this interaction was once considered exclusive, recent studies suggest that SDF-1 may also bind to the CXCR7 receptor. The CXCL12 gene is located on human chromosome 10. Both CXCL12 and CXCR4 exhibit high sequence identity between humans and mice, with 99% and 90% similarity, respectively.
Recombinant Mouse Stromal Cell-Derived Factor-1 alpha is produced in E. coli. It is a non-glycosylated polypeptide chain comprised of 68 amino acids, with a molecular weight of 8 kDa. This protein is purified using proprietary chromatographic methods.
This product appears as a sterile, white powder that has been lyophilized (freeze-dried).
The protein solution undergoes sterile filtration and is then lyophilized from an aqueous solution containing 0.1% Trifluoroacetic Acid (TFA).
To reconstitute the lyophilized Stromal Cell-Derived Factor-1a, it is recommended to dissolve it in sterile 18MΩ-cm H2O at a concentration of at least 100µg/ml. The reconstituted solution can be further diluted in other aqueous solutions.
Lyophilized SDF-1a remains stable at room temperature for up to 3 weeks. However, for long-term storage, it is recommended to store it in a desiccated state below -18°C. Once reconstituted, CXCL12 should be stored at 4°C for 2-7 days. For extended storage, adding a carrier protein like 0.1% HSA or BSA is advisable. Avoid repeated freeze-thaw cycles to maintain protein stability.
The purity of this product is determined by SDS-PAGE analysis and is greater than 95%.
The biological activity of this product is determined by its chemoattractant properties. It can effectively chemoattract human peripheral blood monocytes at concentrations of 50-100 ng/ml. This corresponds to a specific activity of 10,000-20,000 IU/mg.
Product Science Overview
SDF-1α was first identified as the pre-B-cell growth-stimulating factor (PBSF) in the mouse bone marrow-derived stromal cell line, PA6 . It is one of two splice variants produced by cells when stimulated by inflammatory cytokines such as TNF, IL-1, or LPS . The human and mouse versions of SDF-1α share 99% sequence identity . Recombinant mouse SDF-1α is a non-glycosylated protein containing 68 amino acids and has a molecular mass of 7.9 kDa .
SDF-1α signals through the G protein-coupled receptor, CXCR4, to recruit activated leukocytes . This signaling pathway is essential for various biological processes, including immune response, tissue repair, and development. SDF-1α is up-regulated in diseases characterized by tissue hypoxia, such as myocardial infarction and ischemic cardiomyopathy .
Due to its significant role in disease mechanisms, SDF-1α is a potential biomarker for conditions characterized by tissue hypoxia . Researchers have developed novel recombinant antibodies specific to full-length SDF-1α to facilitate its investigation in biomarker studies . These antibodies have been used to quantify full-length SDF-1α in blood, providing insights into its kinetics and role in various conditions .
Recombinant mouse SDF-1α is typically lyophilized from a concentrated sterile solution containing 10 mM acetic acid . The lyophilized product is stable at -20°C, and reconstituted material should be aliquoted and frozen at -20°C for long-term storage . It is recommended to add a carrier protein, such as 0.1% HSA or BSA, for extended storage .
