SDF 1a Human, His

Stromal Cell-Derived Factor-1 alpha Human Recombinant, His Tag
Cat. No.
BT23301
Source
Escherichia Coli.
Synonyms
SDF-1, CXCL12, Pre-B cell growth-stimulating factor, PBSF, hIRH, chemokine (C-X-C motif) ligand 12, SDF1, SDF1A, TPAR1, SCYB12, SDF-1a, TLSF-a.
Appearance
White lyophilized (freeze-dried) powder.
Purity
Greater than 95.0% as determined SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

Stromal Cell-Derived Factor-1 alpha Human Recombinant produced in E.Coli is a non-glycosylated, Polypeptide chain containing 78 amino acids, having a molecular mass of 9.2 kDa.
The SDF-1a is fused to 10 amino acids His-Tag at N-terminus and purified by proprietary chromatographic techniques.

Product Specs

Introduction
SDF-1 (stromal cell-derived factor-1), also known as Chemokine (C-X-C motif) ligand 12 (CXCL12), is a small cytokine belonging to the chemokine family. It exists in two isoforms, SDF-1α/CXCL12a and SDF-1β/CXCL12b, generated by alternative splicing of the same gene. Chemokines are characterized by four conserved cysteines forming two disulfide bonds. CXCL12 proteins belong to the CXC chemokine group, with one amino acid separating the first cysteine pair. CXCL12 exhibits strong chemotactic activity for lymphocytes and plays a crucial role in cell coordination during development, including directing hematopoietic cell migration from the fetal liver to bone marrow during embryogenesis. CXCL12 knockout mice are either lethal before birth or die within an hour of birth. Additionally, CXCL12a influences neuronal electrophysiology. Expression of CXCL12 has been observed in various mouse tissues, including the brain, thymus, heart, lung, liver, kidney, spleen, and bone marrow. CXCL12 binds to its receptor CXCR4 (previously called fusin). While this interaction was initially considered exclusive, recent studies suggest that CXCL12 also binds to the CXCR7 receptor. The CXCL12 gene is located on human chromosome 10. Both CXCL12 and CXCR4 exhibit high sequence identity between humans and mice, with 99% and 90% similarity, respectively.
Description
Recombinant Human Stromal Cell-Derived Factor-1 alpha, produced in E. coli, is a non-glycosylated polypeptide chain comprising 78 amino acids. It has a molecular weight of 9.2 kDa. This SDF-1a protein is fused to a 10 amino acid His-Tag at the N-terminus and purified using proprietary chromatographic techniques.
Physical Appearance
White powder, freeze-dried.
Formulation
The protein solution, at a concentration of 0.5 mg/ml, is filtered through a 0.4 μm filter before being lyophilized. The solution contains 20mM Tris buffer (pH 7.5) and 20mM sodium chloride.
Solubility
To prepare a working solution, add deionized water to achieve a concentration of approximately 0.5 mg/ml. Allow the lyophilized pellet to dissolve completely. Note: This product is not sterile. Before using in cell culture, filter it through an appropriate sterile filter.
Stability
Lyophilized SDF1A remains stable at room temperature for 3 weeks. However, it is recommended to store it desiccated below -18°C. After reconstitution, CXCL12 should be stored at 4°C for 2-7 days. For long-term storage, keep it below -18°C. Adding a carrier protein (0.1% HSA or BSA) is recommended for long-term storage. Avoid repeated freeze-thaw cycles.
Purity
Purity determined by SDS-PAGE is greater than 95%.
Synonyms
SDF-1, CXCL12, Pre-B cell growth-stimulating factor, PBSF, hIRH, chemokine (C-X-C motif) ligand 12, SDF1, SDF1A, TPAR1, SCYB12, SDF-1a, TLSF-a.
Source
Escherichia Coli.
Amino Acid Sequence
MKHHHHHHAS KPVSLSYRCP CRFFESHVAR ANVKHLKILN TPNCALQIVA RLKNNNRQVC IDPKLKWIQE YLEKALNK.

Product Science Overview

Introduction

Stromal Cell-Derived Factor-1 alpha (SDF-1α), also known as C-X-C motif chemokine 12 (CXCL12), is a chemokine protein encoded by the CXCL12 gene in humans . This protein plays a crucial role in various physiological and pathological processes, including embryogenesis, immune surveillance, inflammation response, tissue homeostasis, and tumor growth and metastasis .

Structure and Isoforms

SDF-1α is produced in two main forms, SDF-1α (CXCL12a) and SDF-1β (CXCL12b), through alternative splicing of the same gene . The protein belongs to the intercrine alpha (chemokine CXC) family, characterized by the presence of four conserved cysteines that form two disulfide bonds . The recombinant form of SDF-1α, produced in E. coli, is a non-glycosylated polypeptide chain containing 68 amino acids and has a molecular mass of approximately 8 kDa . The His Tag is a sequence of histidine residues added to the N-terminus of the protein to facilitate purification and detection .

Function and Mechanism

SDF-1α functions as a potent chemoattractant for T-lymphocytes and monocytes but not neutrophils . It activates the C-X-C chemokine receptor CXCR4, inducing a rapid and transient rise in intracellular calcium ions and chemotaxis . Additionally, SDF-1α binds to the atypical chemokine receptor ACKR3, which activates the beta-arrestin pathway and acts as a scavenger receptor for SDF-1 .

Biological Significance

SDF-1α is ubiquitously expressed in many tissues and cell types, including the brain, thymus, heart, lung, liver, kidney, spleen, platelets, and bone marrow . During embryogenesis, it directs the migration of hematopoietic cells from the fetal liver to the bone marrow and the formation of large blood vessels . SDF-1α also functions as a pre-B cell growth factor in the presence of interleukin-7 (IL-7) and is a ligand for CXCR4 or the orphan receptor LESTR/fusin .

Clinical Relevance

Mutations in the CXCL12 gene are associated with resistance to human immunodeficiency virus type 1 (HIV-1) infections . Additionally, CXCL12 signaling has been observed in several cancers, and the gene contains one of 27 single nucleotide polymorphisms (SNPs) associated with an increased risk of coronary artery disease .

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