SDF 1b Mouse

SDF-1β is a non-glycosylated protein composed of 72 amino acids with a molecular weight of approximately 8.5 kDa . It belongs to the CXC chemokine family, characterized by the presence of four conserved cysteines that form two disulfide bonds. The initial pair of cysteines in CXC chemokines are separated by one intervening amino acid .

CXCL12 is ubiquitously expressed in many tissues and cell types, including the brain, thymus, heart, lung, liver, kidney, spleen, platelets, and bone marrow . It is strongly chemotactic for lymphocytes and plays a significant role in the migration and recruitment of activated leukocytes . During embryogenesis, CXCL12 directs the migration of hematopoietic cells from the fetal liver to the bone marrow and aids in the formation of large blood vessels .

SDF-1β signals through the G protein-coupled receptor CXCR4 to recruit activated leukocytes . This signaling pathway is crucial for various immune responses and has been observed in several cancers . Additionally, CXCL12 signaling is involved in the regulation of stem cell homing and retention in the bone marrow .

The biological activity of recombinant mouse SDF-1β is determined by its ability to chemoattract human peripheral T cells stimulated with IL-2 and PHA at concentrations ranging from 10 to 75 ng/mL . This activity highlights its potential role in immune modulation and therapeutic applications.

Recombinant mouse SDF-1β is typically produced in Escherichia coli (E. coli) and is available as a sterile filtered white lyophilized (freeze-dried) powder . It is used primarily for research purposes and is not intended for human, animal, or diagnostic applications . For optimal stability, the lyophilized protein should be stored desiccated below -18°C. Upon reconstitution, it should be aliquoted and stored at the same temperature, with the addition of a carrier protein (0.1% HSA or BSA) for long-term storage .