SDF 1b Human
Stromal Cell Derived Factor-1 Beta Human Recombinant (CXCL12)
Cat. No.
BT23682
Source
Escherichia Coli.
Synonyms
SDF-1, CXCL12, Pre-B cell growth-stimulating factor, PBSF, hIRH, chemokine (C-X-C motif) ligand 12, SDF1, SDF1B, TPAR1, SCYB12, SDF-1b, TLSF-b.
Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Purity
Greater than 98.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
Usage
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Shipped with Ice Packs 
In Stock 
Description
Stromal Cell-Derived Factor-1 beta Human Recombinant produced in E.Coli is a non-glycosylated, Polypeptide chain containing 72 amino acids and having a molecular mass of 8508 Dalton.
The SDF-1b is purified by proprietary chromatographic techniques.
The SDF-1b is purified by proprietary chromatographic techniques.
Product Specs
Introduction
Stromal cell-derived factor-1 (SDF-1), also known as chemokine (C-X-C motif) ligand 12 (CXCL12), is a small cytokine belonging to the chemokine family. It exists in two isoforms, SDF-1α/CXCL12a and SDF-1β/CXCL12b, generated by alternative splicing of the same gene. Chemokines are characterized by four conserved cysteines forming two disulfide bonds. CXCL12 belongs to the CXC chemokine group, where the first two cysteines are separated by one amino acid. CXCL12 exhibits strong chemotactic activity for lymphocytes and plays a crucial role in cell coordination during development. In embryogenesis, it guides hematopoietic cell migration from the fetal liver to the bone marrow. CXCL12 gene knockout mice are lethal before or shortly after birth. Additionally, CXCL12a influences neuronal electrophysiology. CXCL12 expression is observed in various mouse tissues, including the brain, thymus, heart, lung, liver, kidney, spleen, and bone marrow. CXCL12 binds to the CXCR4 receptor, previously known as fusin. While this interaction was considered exclusive, recent studies suggest CXCL12 also binds to the CXCR7 receptor. The CXCL12 gene is located on human chromosome 10. Human and mouse CXCL12 and CXCR4 share high sequence identity at 99% and 90%, respectively.
Description
Recombinant human Stromal Cell-Derived Factor-1 beta, produced in E. coli, is a non-glycosylated polypeptide chain comprising 72 amino acids. With a molecular weight of 8508 Daltons, SDF-1b is purified using proprietary chromatographic techniques.
Physical Appearance
Sterile Filtered White lyophilized powder.
Formulation
The CXCL12 protein was lyophilized from a concentrated (1mg/ml) sterile solution without any additives.
Solubility
To reconstitute the lyophilized Stromal Cell-Derived Factor-1b, it is recommended to dissolve it in sterile 18MΩ-cm H2O at a concentration of at least 100µg/ml. This solution can be further diluted with other aqueous solutions as needed.
Stability
Lyophilized SDF-1b, though stable at room temperature for up to 3 weeks, should be stored desiccated below -18°C. After reconstitution, CXCL12 remains stable at 4°C for 2-7 days. For long-term storage, freezing below -18°C is recommended, preferably with the addition of a carrier protein (0.1% HSA or BSA). Avoid repeated freeze-thaw cycles.
Purity
The purity is determined by two methods and exceeds 98.0%: (a) RP-HPLC analysis and (b) SDS-PAGE analysis.
Biological Activity
The specific activity, determined by chemotaxis assay using PHA and IL-2 activated human peripheral T cells, is measured at a concentration range of 20-80ng/ml. This corresponds to a specific activity of 12,500-50,000IU/mg.
Protein Content
Protein content is quantified using two independent methods: 1. UV spectroscopy at 280 nm, employing an extinction coefficient of 1.06 for a 0.1% (1mg/ml) solution. This value is calculated using the PC GENE computer analysis program for protein sequences (IntelliGenetics). 2. RP-HPLC analysis, employing a standard solution of SDF-1b as a reference standard.
Synonyms
SDF-1, CXCL12, Pre-B cell growth-stimulating factor, PBSF, hIRH, chemokine (C-X-C motif) ligand 12, SDF1, SDF1B, TPAR1, SCYB12, SDF-1b, TLSF-b.
Source
Escherichia Coli.
Amino Acid Sequence
The sequence of the first five N-terminal amino acids was determined and was found to be Lys-Pro-Val-Ser-Leu.
Product Science Overview
Introduction
Stromal Cell-Derived Factor-1 Beta (SDF-1β), also known as C-X-C motif chemokine 12 (CXCL12), is a chemokine protein encoded by the CXCL12 gene on chromosome 10 in humans . This protein plays a crucial role in various physiological and pathological processes, including embryogenesis, immune surveillance, inflammation response, tissue homeostasis, and tumor growth and metastasis .
Structure and Isoforms
CXCL12 is produced in two main isoforms, SDF-1α (CXCL12a) and SDF-1β (CXCL12b), through alternative splicing of the same gene . These isoforms belong to the CXC chemokine family, characterized by the presence of four conserved cysteines that form two disulfide bonds . The initial pair of cysteines in CXC chemokines are separated by one intervening amino acid .
Function and Mechanism
CXCL12 functions as a ligand for the G-protein coupled receptor, chemokine (C-X-C motif) receptor 4 (CXCR4) . This interaction is critical for several cellular functions, including:
- Embryogenesis: Directs the migration of hematopoietic cells from the fetal liver to bone marrow and the formation of large blood vessels .
- Immune Surveillance: Acts as a chemoattractant for lymphocytes, recruiting activated leukocytes .
- Inflammation Response: Induced by proinflammatory stimuli such as lipopolysaccharide (LPS), TNF, or IL-1 .
- Tissue Homeostasis and Repair: Regulates cell migration and behavior during tissue repair and regeneration .
- Tumor Growth and Metastasis: Involved in the migration and behavior of cancer stem cells .
Clinical Significance
Recombinant Production
Human recombinant SDF-1β is produced using recombinant DNA technology, typically in E. coli . The recombinant protein is a non-glycosylated protein, comprised of 72 amino acids with a molecular weight of 8.5 kDa . It is used extensively in research to study its various biological activities and potential therapeutic applications .
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