SDF 1a Rat
Stromal Cell-Derived Factor-1 alpha Rat Recombinant (CXCL12)
Cat. No.
BT23539
Source
Escherichia Coli.
Synonyms
SDF-1, CXCL12, Pre-B cell growth-stimulating factor, PBSF, hIRH, chemokine (C-X-C motif) ligand 12, SDF1, SDF1A, TPAR1, SCYB12, SDF-1a, TLSF-a, 12-O-tetradecanoylphorbol 13-acetate repressed protein 1, Thymic lymphoma cell-stimulating factor, TLSF.
Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Purity
Greater than 97.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
Usage
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Shipped with Ice Packs 
In Stock 
Description
Stromal Cell-Derived Factor-1 alpha Rat Recombinant produced in E.Coli is a non-glycosylated, Polypeptide chain containing 68 amino acids and having a molecular mass of 7.9 kDa.
The SDF-1a is purified by proprietary chromatographic techniques.
The SDF-1a is purified by proprietary chromatographic techniques.
Product Specs
Introduction
SDF-1, or stromal cell-derived factor-1, is a chemokine officially known as Chemokine (C-X-C motif) ligand 12 (CXCL12). It exists in two isoforms, SDF-1α/CXCL12a and SDF-1β/CXCL12b, generated by alternative splicing of the CXCL12 gene. Chemokines are characterized by four conserved cysteines forming two disulfide bonds. CXCL12 belongs to the CXC chemokine group, with one amino acid separating the first cysteine pair. Highly chemotactic for lymphocytes, CXCL12 plays a crucial role in cell coordination during development, including directing hematopoietic cell migration from the fetal liver to bone marrow during embryogenesis. CXCL12 knockout mice are embryonic lethal or die within an hour of birth. Additionally, CXCL12a influences neuronal electrophysiology. CXCL12 expression is observed in various murine tissues, including the brain, thymus, heart, lung, liver, kidney, spleen, and bone marrow. CXCL12 binds to the CXCR4 receptor, previously known as fusin. While this interaction was considered exclusive, recent studies suggest CXCL12 also binds to the CXCR7 receptor. The CXCL12 gene is located on human chromosome 10. Both CXCL12 and CXCR4 exhibit high sequence identity between humans and mice, with 99% and 90% similarity, respectively.
Description
Recombinant Rat Stromal Cell-Derived Factor-1 alpha is produced in E. coli. It is a non-glycosylated polypeptide chain consisting of 68 amino acids, with a molecular weight of 7.9 kDa. This SDF-1a product undergoes purification using proprietary chromatographic techniques.
Physical Appearance
Sterile white lyophilized powder.
Formulation
The CXCL12 protein was lyophilized from a sterile solution at a concentration of 1 mg/ml in 20mM Phosphate buffer with a pH of 7.4 and 150mM NaCl.
Solubility
To reconstitute the lyophilized SDF-1a, it is recommended to dissolve it in sterile 18 MΩ-cm H2O to a concentration of at least 100 µg/ml. This solution can be further diluted in other aqueous solutions as needed.
Stability
Lyophilized SDF-1a remains stable at room temperature for up to 3 weeks. However, for long-term storage, it is recommended to store it desiccated at a temperature below -18°C. After reconstitution, CXCL12 should be stored at 4°C for 2-7 days. For extended storage, it is advisable to store it below -18°C. The addition of a carrier protein, such as 0.1% HSA or BSA, is recommended for long-term storage. It is important to avoid repeated freeze-thaw cycles.
Purity
The purity of this product is greater than 97.0%, as determined by: (a) RP-HPLC analysis and (b) SDS-PAGE analysis.
Biological Activity
The specific activity of this product is determined by its ability to chemoattract human peripheral blood monocytes. At concentrations of 50-100 ng/ml, it demonstrates a specific activity of 10,000-20,000 IU/mg.
Synonyms
SDF-1, CXCL12, Pre-B cell growth-stimulating factor, PBSF, hIRH, chemokine (C-X-C motif) ligand 12, SDF1, SDF1A, TPAR1, SCYB12, SDF-1a, TLSF-a, 12-O-tetradecanoylphorbol 13-acetate repressed protein 1, Thymic lymphoma cell-stimulating factor, TLSF.
Source
Escherichia Coli.
Amino Acid Sequence
KPVSLSYRCP CRFFESHVAR ANVKHLKILN TPNCALQIVA RLKSNNRQVC IDPKLKWIQE YLDKALNK.
Product Science Overview
Structure and Function
SDF-1α is a small protein with a molecular weight of approximately 7.9 kDa, consisting of 68 amino acid residues . It is produced by stromal cells and functions primarily through its interaction with the CXCR4 receptor, a G-protein-coupled receptor. This interaction is essential for the chemotaxis of lymphocytes and macrophages, migration of hematopoietic cells from the fetal liver to the bone marrow, and the formation of large blood vessels .
Biological Significance
- Immune System: SDF-1α is critical in the immune system, where it acts as a chemoattractant for lymphocytes and macrophages . It helps in the migration and homing of these cells to sites of inflammation or injury.
- Tissue Regeneration: SDF-1α is upregulated in response to tissue hypoxia, such as during myocardial infarction or ischemic cardiomyopathy . It promotes tissue repair and regeneration by recruiting stem cells and progenitor cells to the damaged area.
- Cancer Metastasis: The SDF-1α/CXCR4 axis is implicated in the metastasis of various cancers, including breast cancer . It facilitates the migration of cancer cells to distant organs, contributing to the spread of the disease.
Recombinant SDF-1α
Recombinant SDF-1α, such as the rat recombinant version, is produced using E. coli expression systems . This recombinant protein is used extensively in research to study its biological functions and potential therapeutic applications. It is highly purified, with a purity of ≥ 98% as determined by SDS-PAGE gel and HPLC analyses .
Applications in Research
- Chemotaxis Assays: Recombinant SDF-1α is used in chemotaxis assays to study the migration of immune cells and cancer cells in response to chemokine gradients .
- Tissue Engineering: It is utilized in tissue engineering and regenerative medicine to enhance the recruitment of stem cells to sites of injury or disease .
- Cancer Research: Researchers use recombinant SDF-1α to investigate its role in cancer metastasis and to develop potential therapeutic strategies targeting the SDF-1α/CXCR4 axis .
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