SDF 1a Mouse, His

Stromal Cell-Derived Factor-1 alpha (CXCL12), Mouse Recombinant, His Tag
Cat. No.
BT23468
Source
Escherichia Coli.
Synonyms
SDF-1, CXCL12, Pre-B cell growth-stimulating factor, PBSF, hIRH, chemokine (C-X-C motif) ligand 12, SDF1, SDF1A, TPAR1, SCYB12, SDF-1a, TLSF-a, 12-O tetradecanoylphorbol 13-acetate repressed protein 1, Thymic lymphoma cell stimulating factor, TLSF.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 95.0% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

SDF 1a Mouse Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 91 amino acids (22-89 a.a) and having a molecular mass of 10.4kDa. SDF 1a is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
Stromal cell-derived factor-1 (SDF-1), also known as chemokine (C-X-C motif) ligand 12 (CXCL12), is a small cytokine belonging to the chemokine family. It exists in two isoforms, SDF-1α/CXCL12a and SDF-1β/CXCL12b, generated by alternative splicing of the same gene. Chemokines are characterized by the presence of four conserved cysteine residues forming two disulfide bonds. CXCL12 proteins belong to the CXC chemokine group, distinguished by one amino acid separating their first cysteine pair. CXCL12 exhibits strong chemotactic activity for lymphocytes and plays a crucial role in cell coordination during development. During embryogenesis, it guides the migration of hematopoietic cells from the fetal liver to the bone marrow. Mice lacking the CXCL12 gene die before birth or within an hour of birth. Furthermore, CXCL12a influences neuronal electrophysiology. In mice, CXCL12 expression is observed in various tissues, including the brain, thymus, heart, lung, liver, kidney, spleen, and bone marrow. The receptor for CXCL12 is CXCR4, previously known as fusin. While the CXCL12-CXCR4 interaction was once thought to be exclusive, recent studies suggest that CXCL12 can also bind to the CXCR7 receptor. The gene encoding CXCL12 is located on human chromosome 10. Both CXCL12 and CXCR4 exhibit high sequence identity between humans and mice, with 99% and 90% similarity, respectively.
Description
SDF-1a Mouse Recombinant, produced in E. coli, is a monomeric, non-glycosylated polypeptide chain comprising 91 amino acids (22-89 a.a.). It has a molecular mass of 10.4 kDa. The recombinant protein consists of the SDF-1a sequence fused to a 23 amino acid His-tag at the N-terminus. Purification is achieved using proprietary chromatographic techniques.
Physical Appearance
Clear, colorless solution, sterile-filtered.
Formulation
The SDF-1a protein is supplied in a solution at a concentration of 0.25 mg/ml. The solution contains Phosphate Buffered Saline (pH 7.4) and 10% glycerol.
Stability
For short-term storage (2-4 weeks), the product can be stored at 4°C. For extended storage, it is recommended to freeze the product at -20°C. To ensure long-term stability during frozen storage, adding a carrier protein (0.1% HSA or BSA) is advisable. Avoid repeated freeze-thaw cycles.
Purity
The purity of the SDF-1a Mouse Recombinant protein is greater than 95%, as determined by SDS-PAGE analysis.
Synonyms
SDF-1, CXCL12, Pre-B cell growth-stimulating factor, PBSF, hIRH, chemokine (C-X-C motif) ligand 12, SDF1, SDF1A, TPAR1, SCYB12, SDF-1a, TLSF-a, 12-O tetradecanoylphorbol 13-acetate repressed protein 1, Thymic lymphoma cell stimulating factor, TLSF.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSKPVSLSY RCPCRFFESH IARANVKHLK ILNTPNCALQ IVARLKNNNR QVCIDPKLKW IQEYLEKALN K.

Product Science Overview

Introduction

Stromal Cell-Derived Factor-1 alpha (SDF-1α), also known as C-X-C motif chemokine 12 (CXCL12), is a chemokine protein that plays a crucial role in various physiological and pathological processes. It is a member of the CXC chemokine family and is known for its ability to regulate the migration, proliferation, differentiation, and survival of various cell types, including hematopoietic stem cells, B cells, and T cells .

Gene and Protein Structure

The CXCL12 gene is located on chromosome 10 in humans and on chromosome 6 in mice . This gene produces multiple isoforms through alternative splicing, with SDF-1α and SDF-1β being the most studied variants . The protein structure of CXCL12 is characterized by the presence of four conserved cysteines that form two disulfide bonds, which are essential for its biological activity .

Function and Mechanism of Action

CXCL12 exerts its effects by binding to the G-protein coupled receptor CXCR4, which triggers a cascade of intracellular signaling pathways . This interaction is critical for the chemotactic activity of CXCL12, guiding the migration of cells to specific tissues. CXCL12 is strongly chemotactic for lymphocytes and plays a vital role in immune surveillance, inflammation response, tissue homeostasis, and tumor growth and metastasis .

Biological Significance

In mice, CXCL12 is expressed in various tissues, including the brain, thymus, heart, lung, liver, kidney, spleen, platelets, and bone marrow . During embryogenesis, CXCL12 directs the migration of hematopoietic cells from the fetal liver to the bone marrow and contributes to the formation of large blood vessels . Additionally, CXCL12 signaling has been implicated in several cancers and is associated with increased risk of coronary artery disease .

Recombinant CXCL12 (Mouse, His Tag)

Recombinant CXCL12 (Mouse, His Tag) is a laboratory-produced version of the mouse CXCL12 protein, engineered to include a His tag for easy purification and detection. This recombinant protein is widely used in research to study the biological functions of CXCL12 and its interactions with CXCR4. It is particularly valuable in experiments involving cell migration, proliferation, and differentiation .

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