SCF Human, Sf9
Stem Cell Factor Human Recombinant, Sf9
Cat. No.
BT2247
Source
Sf9, Insect Cells.
Synonyms
Kit ligand Precursor, C-kit ligand, SCF, Mast cell growth factor, MGF, SF, KL-1, Kitl, DKFZp686F2250.
Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Purity
Greater than 98.0% as determined by analysis by SDS-PAGE.
Usage
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description
Stem Cell Factor Human Recombinant produced in insect cells is a single, glycosylated polypeptide chain containing 165 amino acids and having a molecular mass of 18409 Dalton.
The SCF is fused to a C-terminal His-tag (6xHis) and purified by proprietary chromatographic techniques.
The SCF is fused to a C-terminal His-tag (6xHis) and purified by proprietary chromatographic techniques.
Product Specs
Introduction
Stem cell factor/KIT ligand (SCF), also known as "steel factor" or "c-kit ligand," is a cytokine that binds to CD117 (c-Kit). It exists in two forms: cell surface bound and soluble (or free) SCF, produced by metalloprotease cleavage of the bound form. SCF is crucial for the survival, proliferation, and differentiation of hematopoietic stem cells and progenitor cells. It plays a role in the development of erythrocyte precursors by converting BFU-E (burst-forming unit-erythroid) cells into CFU-E (colony-forming unit-erythroid) cells.
Description
Recombinant Human Stem Cell Factor, expressed in insect cells, is a single, glycosylated polypeptide chain with 165 amino acids and a molecular weight of 18.4 kDa. This SCF protein has a C-terminal His-tag (6xHis) and is purified using proprietary chromatographic techniques.
Physical Appearance
Sterile, white lyophilized (freeze-dried) powder.
Formulation
The protein is supplied in 1x PBS with a pH of 7.4.
Solubility
Reconstitute the lyophilized Stem Cell Factor in 10 mM acetic acid to a concentration of at least 100 µg/ml. This solution can be further diluted in other aqueous solutions.
Stability
Lyophilized KIT ligand is stable at room temperature for 3 weeks but should be stored desiccated below -18°C. Reconstituted SCF should be stored at 4°C for 2-7 days or below -18°C for long-term storage. For long-term storage, adding a carrier protein (0.1% HSA or BSA) is recommended. Avoid freeze-thaw cycles.
Purity
Purity is greater than 98.0% as determined by SDS-PAGE analysis.
Biological Activity
The ED50, measured by the dose-dependent stimulation of Human TF-1 cells, is typically 1-5 ng/ml.
Protein Content
Protein concentration was determined using two independent methods: 1) UV spectroscopy at 280 nm with an absorbance value of 0.52 as the extinction coefficient for a 0.1% (1 mg/ml) solution. This value is calculated by the PC GENE computer analysis program for protein sequences (IntelliGenetics). 2) RP-HPLC analysis using a standard solution of Stem Cell Factor as a reference standard.
Synonyms
Kit ligand Precursor, C-kit ligand, SCF, Mast cell growth factor, MGF, SF, KL-1, Kitl, DKFZp686F2250.
Source
Sf9, Insect Cells.
Product Science Overview
Biological Significance
SCF is a pleiotropic cytokine, meaning it has multiple effects on different cell types. It is involved in:
- Hematopoiesis: SCF is vital for the survival, proliferation, and differentiation of hematopoietic stem cells and progenitor cells. It acts at multiple levels of the hematopoietic hierarchy, promoting the formation of various blood cell lineages .
- Stem Cell Maintenance: SCF supports the maintenance and self-renewal of stem cells, ensuring a steady supply of these cells for tissue regeneration and repair .
- Gametogenesis: SCF is essential for the development and maturation of germ cells, playing a role in both spermatogenesis and oogenesis .
- Mast Cell Development: SCF is crucial for the development, migration, and function of mast cells, which are involved in immune responses and allergic reactions .
- Melanogenesis: SCF influences the production of melanin in melanocytes, affecting skin pigmentation .
Forms of SCF
Recombinant SCF Production in Sf9 Cells
Human Recombinant SCF (Sf9) is produced using the Sf9 insect cell expression system. This system involves the following steps:
- Gene Cloning: The gene encoding human SCF is cloned into a suitable expression vector.
- Transfection: The expression vector is introduced into Sf9 insect cells, which are capable of producing recombinant proteins.
- Protein Expression: The transfected Sf9 cells express the SCF protein, which is then secreted into the culture medium.
- Purification: The recombinant SCF is purified using chromatographic techniques to obtain a high-purity product .
Applications
Recombinant SCF has several applications in research and medicine:
- Stem Cell Research: SCF is used to study the behavior of stem cells, including their proliferation, differentiation, and self-renewal.
- Hematopoietic Stem Cell Expansion: SCF is used to expand hematopoietic stem cells in vitro, which can be beneficial for bone marrow transplantation and other therapeutic applications .
- Mast Cell Studies: SCF is used to investigate the development and function of mast cells, which are important in allergy and immune response research .
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