SCF Mouse
Stem Cell Factor Mouse Recombinant
Cat. No.
BT2336
Source
Escherichia Coli.
Synonyms
Kit ligand Precursor, C-kit ligand, SCF, Mast cell growth factor, MGF, SF, KL-1, Kitl, DKFZp686F2250, Hematopoietic growth factor KL, Steel factor.
Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Purity
Greater than 98.0% as determined by:
(a) Analysis by SEC-HPLC.
(b) Analysis by SDS-PAGE.
(a) Analysis by SEC-HPLC.
(b) Analysis by SDS-PAGE.
Usage
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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In Stock 
Description
Stem Cell Factor Mouse Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 165 amino acids and having a molecular mass of 18309 Dalton.
The SCF is purified by proprietary chromatographic techniques.
The SCF is purified by proprietary chromatographic techniques.
Product Specs
Introduction
Stem cell factor / KIT ligand (SCF), also known as "steel factor" or "c-kit ligand", is a cytokine that binds to CD117 (c-Kit). It exists in two forms: cell surface bound and soluble (or free), the latter generated by metalloprotease cleavage of the former. SCF is crucial for hematopoietic stem cell and progenitor cell survival, proliferation, and differentiation. Notably, it drives the differentiation of burst-forming unit-erythroid (BFU-E) cells, the earliest erythrocyte precursors, into colony-forming unit-erythroid (CFU-E) cells.
Description
Recombinant Mouse Stem Cell Factor, produced in E. coli, is a single, non-glycosylated polypeptide chain comprising 165 amino acids. With a molecular mass of 18.3 kDa, this purified protein is obtained through proprietary chromatographic techniques.
Physical Appearance
Sterile, white lyophilized powder.
Formulation
Lyophilized from a 1 mg/mL solution in water containing 0.02% sodium bicarbonate.
Solubility
Reconstitute the lyophilized Stem Cell Factor in sterile 18 MΩ-cm H₂O to a concentration of at least 100 µg/mL. This solution can be further diluted in other aqueous solutions.
Stability
Lyophilized KIT ligand remains stable at room temperature for 3 weeks but should be stored desiccated below -18°C. Upon reconstitution, store SCF at 4°C for 2-7 days. For long-term storage, add a carrier protein (0.1% HSA or BSA) and store below -18°C. Avoid freeze-thaw cycles.
Purity
Purity exceeding 98.0% as determined by: (a) Size-exclusion high-performance liquid chromatography (SEC-HPLC) analysis and (b) Sodium dodecyl-sulfate polyacrylamide gel electrophoresis (SDS-PAGE) analysis.
Biological Activity
The ED₅₀, determined by dose-dependent stimulation of the human TF-1 cell line, is less than 10 ng/mL. This corresponds to a specific activity of 1 x 10⁵ IU/mg.
Protein Content
Protein quantification was performed using two independent methods: (1) UV spectroscopy at 280 nm employing an absorbency value of 0.52 as the extinction coefficient for a 0.1% (1 mg/mL) solution, calculated using the PC GENE computer analysis program (IntelliGenetics), and (2) Reverse-phase high-performance liquid chromatography (RP-HPLC) analysis using a standard solution of Stem Cell Factor as a reference standard.
Synonyms
Kit ligand Precursor, C-kit ligand, SCF, Mast cell growth factor, MGF, SF, KL-1, Kitl, DKFZp686F2250, Hematopoietic growth factor KL, Steel factor.
Source
Escherichia Coli.
Amino Acid Sequence
MKEICGNPVT DNVKDITKLV ANLPNDYMIT LNYVAGMDVL PSHCWLRDMV IQLSLSLTTL LDKFSNISEG LSNYSIIDKL GKIVDDLVLC MEENAPKNIK ESPKRPETRS FTPEEFFSIF NRSIDAFKDF MVASDTSDCV LSSTLGPEKD SRVSVTKPFM LPPVA.
Product Science Overview
Structure and Forms
Biological Functions
SCF is essential for:
- Hematopoiesis: The formation of blood cells.
- Spermatogenesis: The development of sperm cells.
- Melanogenesis: The production of melanin in the skin .
In mice, SCF is particularly important during fetal development. It is produced by stromal cells in various tissues, including the fetal liver, bone marrow, thymus, central nervous system, keratinocytes, and gut mucosa . SCF functions as a chemotactic and chemokinetic factor, guiding the movement and activity of cells .
Mechanism of Action
Applications in Research
Recombinant SCF, particularly mouse recombinant SCF, is widely used in research to:
Clinical Relevance
SCF and its receptor c-KIT are also implicated in various clinical conditions. For instance, mutations in the c-KIT receptor can lead to disorders such as piebaldism, a condition characterized by patches of unpigmented skin and hair . Additionally, SCF signaling is involved in the proliferation and invasiveness of certain tumor cells .
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