SCF Human, HEK
Description
The SCF is purified by proprietary chromatographic techniques.
Product Specs
Product Science Overview
Recombinant human SCF is often produced using Human Embryonic Kidney (HEK) 293 cells. The HEK293 cell line is derived from human embryonic kidney cells and has been widely used in biological research due to its high transfection efficiency and ability to produce large quantities of recombinant proteins . The use of HEK293 cells for the production of SCF ensures human-like glycosylation and proper protein folding, which are critical for the biological activity of the cytokine .
Recombinant human SCF expressed in HEK293 cells typically has a molecular mass of 35-45 kDa . The protein is manufactured without the use of serum, which minimizes the risk of contamination and ensures a higher specific activity of the protein. The human cell expression system allows for the production of SCF with post-translational modifications that are similar to those found in naturally occurring human proteins .
SCF binds to the c-Kit receptor (CD117), a type of receptor tyrosine kinase, and activates several intracellular signaling pathways, including the MAPK, PI3K, and PLCγ pathways . These signaling pathways are involved in various cellular processes such as cell survival, proliferation, differentiation, and migration. SCF is particularly important for the maintenance of hematopoietic stem cells in the bone marrow niche and plays a role in the mobilization of these cells into the peripheral blood.
Due to its critical role in hematopoiesis, SCF is widely used in research and clinical applications. It is used to support the growth and differentiation of hematopoietic stem cells in vitro, which is essential for various experimental and therapeutic purposes. SCF is also used in the expansion of stem cells for transplantation and in the treatment of certain hematological disorders.
In addition to its role in hematopoiesis, SCF has been implicated in the development and progression of various cancers. Elevated levels of SCF and its receptor c-Kit have been observed in several types of tumors, including gastrointestinal stromal tumors (GISTs), mastocytosis, and certain leukemias . As a result, SCF and c-Kit are considered potential targets for cancer therapy.
