SCF Rat

The recombinant form of rat SCF is typically produced in Escherichia coli (E. coli) and is a non-glycosylated protein containing 165 amino acids, with a molecular weight of approximately 18.4 kDa . The protein is often provided in a lyophilized (freeze-dried) form and requires reconstitution before use. It is recommended to reconstitute the lyophilized product with sterile 10 mM acetic acid (AcOH) at a concentration of 0.1 mg/ml, which can be further diluted into other aqueous solutions .

SCF is known for its ability to induce the proliferation of TF-1 cells, a human erythroleukemia cell line, which is typically observed at concentrations below 10 ng/ml . This activity underscores its importance in hematopoiesis, the process of forming new blood cells. SCF also plays a significant role in spermatogenesis (the production of sperm) and melanogenesis (the production of melanin in the skin) .

Recombinant SCF is widely used in research settings, particularly in studies involving stem cell biology and hematopoiesis. It is crucial for maintaining the viability and proliferation of hematopoietic stem cells in culture. For long-term storage, it is recommended to add a carrier protein, such as 0.1% human serum albumin (HSA) or bovine serum albumin (BSA), to prevent protein degradation .

The discovery and utilization of SCF have significantly advanced our understanding of stem cell biology and the mechanisms underlying blood cell formation. The ability of SCF to support the survival and proliferation of hematopoietic stem cells makes it a valuable tool in both basic research and potential therapeutic applications.