s100bb Bovine
s100bb Bovine homodimer
Cat. No.
BT1526
Source
Bovine brain tissue.
Synonyms
Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Purity
Greater than 95.0% .
Usage
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description
s100bb Bovine is suitable for use as a standard in immunoassay and as an immunogen for antiserum production.
Product Specs
Description
s100bb Bovine is suitable for use as a standard in immunoassay and as an immunogen for antiserum production.
Physical Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation
s100bb was lyophilized from 5mM Tris-HCl, pH 7.5, 2mM EDTA and 5mM 2-mercaptoethanol.
Solubility
It is recommended to reconstitute the lyophilized s100bb in sterile 18MΩ-cm H2O containing 5mM 2- mercaptoethanol not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Stability
Lyophilized s100bb homodimer although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution s100bb should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Purity
Greater than 95.0%.
Source
Bovine brain tissue.
Product Science Overview
Structure and Properties
- Source: S100BB is isolated from bovine brain tissue .
- Purity: The protein is highly purified, with a purity level exceeding 95% .
- Electrophoresis: After native gel electrophoresis by the Ornstein-Davis method, S100BB is presented as a single band corresponding to the dimeric protein .
- Concentration Determination: The concentration of S100BB is determined using the Lowry method, with Bovine Serum Albumin (BSA) as a standard .
Applications
S100BB homodimer is widely used in research and clinical settings due to its various applications:
Storage and Handling
- Lyophilization: The protein is lyophilized from a solution containing 5 mM Tris-HCl (pH 7.5), 5 mM 2-mercaptoethanol, and 2 mM EDTA .
- Reconstitution: It is recommended to reconstitute the lyophilized product with deionized water containing 5 mM 2-mercaptoethanol to its initial concentration .
- Storage Conditions: Both lyophilized and reconstituted forms should be stored at -20°C, with an allowable range of -15°C to -30°C .
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