Introduction
S100b, a member of the S100 protein family, plays a crucial role in various cellular processes. This family comprises EF-hand calcium-binding proteins, often existing as dimers of 20 identified S100 monomers. The S100B homodimer is found in cells of the central nervous system, glial cells, and specific peripheral cells like Schwann cells, melanocytes, adipocytes, and chondrocytes. Located in the cytoplasm or nucleus of diverse cells, S100 proteins regulate cellular processes such as cell cycle progression and differentiation. The S100 gene family, consisting of at least 13 members, clusters on chromosome 1q21, except for S100b, located at 21q22.3. Serum S100B levels can help monitor brain injury and malignant melanoma. S100b proteins are involved in neurite extension, melanoma cell proliferation, stimulation of calcium fluxes, inhibition of PKC-mediated phosphorylation, astrocytosis, axonal proliferation, and microtubule assembly inhibition. Chromosomal rearrangements and altered S100b gene expression are implicated in neurological, neoplastic, and other diseases, including Alzheimer's disease, Down's syndrome, epilepsy, amyotrophic lateral sclerosis, melanoma, and type I diabetes.
Description
S100b Human Recombinant, produced in E. coli, is a single, non-glycosylated polypeptide chain consisting of 92 amino acids (1-92 a.a.) with a molecular mass of 10.7 kDa. The protein is purified using proprietary chromatographic techniques.
Physical Appearance
The product appears as a clear, colorless solution without any particulate matter.
Formulation
The S100b protein solution is provided at a concentration of 1 mg/ml. It is formulated in a buffer containing Phosphate Buffered Saline (pH 7.4) and 10% glycerol.
Stability
For optimal storage, the product should be kept refrigerated at 4°C. At this temperature, the entire vial content can be used within 2-4 weeks. For extended storage periods, freezing at -20°C is recommended. Adding a carrier protein like HSA or BSA (0.1%) is advisable for long-term storage. Repeated freezing and thawing should be avoided to maintain product integrity.
Purity
The purity of the S100b protein is greater than 95.0%, as determined by SDS-PAGE analysis.
Synonyms
S100
Calcium Binding Protein B, S-100 Protein Subunit Beta, S100 Calcium Binding
Protein, Beta (Neural), S100 Calcium-Binding Protein, Beta (Neural), S-100
Calcium-Binding Protein, Beta Chain, S100 Calcium-Binding Protein B, S-100
Protein Beta Chain, S100beta, S100-B, S100, NEF.
Amino Acid Sequence
MSELEKAMVA
LIDVFHQYSG REGDKHKLKK SELKELINNE LSHFLEEIKE QEVVDKVMET LDNDGDGECD FQEFMAFVAM
VTTACHEFFE HE.