S100b Rhesus Macaque

S100 Calcium Binding Protein B Rhesus Macaque Recombinant
Cat. No.
BT1428
Source
Escherichia Coli.
Synonyms
Protein S100-B, S100 calcium-binding protein B, S-100 protein subunit beta, S-100 protein beta chain, S100B, NEF, S100, S100beta.
Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Purity
Greater than 97.0% as determined by SDS-PAGE and HPLC analyses.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

S100B Rhesus Macaque Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 91 amino acids and having a molecular mass of 10.7kDa.
The S100B Rhesus Macaque is purified by proprietary chromatographic techniques.

Product Specs

Introduction
S100b, a member of the S100 protein family, is an EF-hand calcium-binding protein that primarily exists as a dimer. The S100 protein family consists of 20 identified individual S100 monomers. The S100B homodimer is found in cells within the central nervous system, glial cells, and specific peripheral cells like Schwann cells, melanocytes, adipocytes, and chondrocytes. S100 proteins are located in either the cytoplasm or nucleus of a wide variety of cells and play a role in regulating cellular processes like cell cycle progression and differentiation. The S100 gene family comprises at least 13 members clustered on chromosome 1q21, with the exception of S100b, which is located at 21q22.3. Serum S100B levels can be measured to assess the severity of brain injury and malignant melanoma. S100b proteins may be involved in various functions, including neurite extension, melanoma cell proliferation, stimulation of calcium ion (Ca2+) fluxes, inhibition of protein kinase C (PKC)-mediated phosphorylation, astrocytosis, axonal proliferation, and microtubule assembly inhibition. Chromosomal rearrangements and altered S100b gene expression are associated with several neurological, neoplastic, and other diseases, such as Alzheimer's disease, Down's syndrome, epilepsy, amyotrophic lateral sclerosis, melanoma, and type I diabetes.
Description
S100B Rhesus Macaque Recombinant, produced in E. coli, is a single, non-glycosylated polypeptide chain comprising 91 amino acids with a molecular weight of 10.7 kDa. The protein is purified using proprietary chromatographic techniques.
Physical Appearance
Sterile Filtered White lyophilized (freeze-dried) powder
Formulation
Lyophilized from a 0.2µm filtered concentrated solution in phosphate-buffered saline (PBS) at pH 7.4.
Solubility
Reconstitute the lyophilized S100B Rhesus Macaque in sterile 18 MΩ-cm H2O to a concentration of at least 100 µg/ml. This solution can then be further diluted into other aqueous solutions.
Stability
Lyophilized S100B Rhesus Macaque is stable at room temperature for 3 weeks; however, it should be stored desiccated below -18°C. After reconstitution, store S100B Rhesus Macaque at 4°C for 2-7 days. For long-term storage, store below -18°C. Avoid repeated freeze-thaw cycles.
Purity
Greater than 97.0% purity as determined by SDS-PAGE and HPLC analyses.
Synonyms
Protein S100-B, S100 calcium-binding protein B, S-100 protein subunit beta, S-100 protein beta chain, S100B, NEF, S100, S100beta.
Source
Escherichia Coli.
Amino Acid Sequence
SELEKAMVAL IDVFHQYSGR EGDKHKLKKS ELKELINNEL SHFLEEIKEQ EVVDKVMETL DSDGDGECDF QEFMAFVAMV TTACHEFFEH E

Product Science Overview

Introduction

S100 Calcium Binding Protein B (S100B) is a member of the S100 protein family, characterized by its ability to bind calcium and zinc ions. This protein family is part of the EF-hand (helix E-loop-helix F) subgroup, which is known for its helix-loop-helix structural motif that facilitates calcium binding . S100B plays a crucial role in the central nervous system and is associated with various neurological conditions, including Alzheimer’s and Parkinson’s diseases .

Source and Expression

The recombinant form of S100B from Rhesus Macaque is typically expressed in Escherichia coli (E. coli) systems. The protein is produced as a single non-glycosylated polypeptide chain consisting of 91 amino acids . The amino acid sequence of the recombinant protein is as follows:

SELEKAMVAL IDVFHQYSGR EGDKHKLKKS ELKELINNEL SHFLEEIKEQ EVVDKVMETL DSDGDGECDF QEFMAFVAMV TTACHEFFEH E
Purification and Quality Control

The recombinant S100B protein is purified using High-Performance Liquid Chromatography (HPLC) and is subjected to stringent quality control measures, including SDS-PAGE and Western Blot analyses . These methods ensure that the protein is highly pure, with a purity level exceeding 97% . The endotoxin level is kept below 1 EU/µg, as determined by the Limulus Amebocyte Lysate (LAL) method .

Biological Activity

S100B is biologically active and has been validated on appropriate cell lines for its bioactivity . It plays a significant role in the development and function of the central nervous system. Additionally, S100B serves as a biomarker for brain injury and is involved in various neurological diseases .

Storage and Stability

The recombinant S100B protein should be stored at -20°C to -70°C for long-term storage. After reconstitution, it can be stored at 2°C to 8°C for up to one month . Proper storage conditions are essential to maintain the protein’s stability and biological activity.

Applications

Recombinant S100B protein is used in various research applications, including studies on neurological diseases, brain injury, and calcium signaling pathways. It is also utilized in drug discovery and development processes .

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